Browsing by Autor "Solano, Marco"

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DIAGNÓSTICO DE LEISHMANIASIS UTILIZANDO MEDIO DE CULTIVO TSTB EN PACIENTES DE TROPICO DE COCHABAMBA
(Gac Med Bol, 2005) Bermúdez, Hernán; Solano, Marco; Torrico Rojas, Mary Cruz; Carballo Montero, Mauricio; Lafuente Covarrubias, Omar; Lara Arias, María del Pilar; Paredes Torrico, Paola
Se realizó un estudio prospectivo, analítico y experimental para determinar el porcentaje de sensibilidad de un nuevo medio de cultivo para el diagnóstico de Leishmania, denominado TSTB (Torrico-Solano-Torrico-Bermúdez). Se obtuvieron las muestras por aspirado de úlceras con sospecha clínica de Leishmaniasis de pacientes provenientes del trópico de Cochabamba. Los objetivos planteados fueron determinar el porcentaje de sensibilidad del cultivo ya mencionado y analizar el crecimiento de parásitos de Leishmania. Como resultados se obtuvo un 90% de sensibilidad mediante este método diagnóstico y una mínima contaminación por hongos (mohos y levaduras); además, un cambio de coloración en el medio de cultivo debido al crecimiento y multiplicación de los parásitos por consumo de los nutrientes.
Efecto antibacteriano de la miel de abeja en diferentes concentraciones frente a staphylococcus aureus
(Rev Cient Cienc Méd, 2016) Becerra Torrejon, Darwin Jose; Cabrera Ureña, Janette Claudia; Solano, Marco
El Staphylococcus aureus es una bacteria grampositiva, catalasa-positiva dispuestas en racimos, los factores de virulencia incluyen componentes estructurales que facilitan la adherencia a los tejidos del hospedador, Específicamente a la miel se le han reconocido propiedades medicinales desde la antigüedad: como tratamiento terapéutico contra infecciones causadas por bacterias en heridas abiertas, ulceras, quemaduras e infecciones oculares. Objetivo: Determinar la actividad antibacteriano de la miel de abeja en sus diferentes concentraciones frente al Staphylococcus aureus. El estudio es Experimental, prospectivo y transversal. Se basó en la utilización de 6 cultivos de Staphylococcus aureus en caldo nutritivo con una determinadas cantidad de colonias a las cuales se aplicó miel de abeja en concentraciones de 30%, 60% y 100% que se dejó incubar por 24 horas para luego observar el efecto antibacteriano a través de un cultivo en agar sangre en otras 24 hrs. Comprobamos que la actividad bactericida de la miel frente al Staphylococcus Aureus es muy efectiva siendo esta una alternativa de tratamiento frente a dicha bacteria y se pudo constatar que la miel de abeja a una mayor concentración produce mayor efecto antibacteriano sobre el Staphylococcus aureus.
[Effects of maternal infection with Trypanosoma cruzi in pregnancy development and in the newborn infant].
(2005) Torrico, Faustino; Castro, Mildred; Solano, Marco; Rodriguez, Patricia; Torrico, Mary-Cruz; Truyens, Carine; Carlier, Yves
In the endemic regions of Bolivia the infection of the feminine population in fertile age by T. cruzi is frequent (20 to 50 % of the women in fertile age) and the rate of fetal maternal transmission is of approximately 5%. A great percentage of infected women do not transmit the infection to the fetus. The intention of the present study carried out at the Maternal-Infantile Hospital Germán Urquidi of Cochabamba (Bolivia) is to contribute to the knowledge regarding the pregnancy and birth of a newborn of Chagas infected women who do not transmit the infection to the fetus. 2124 mothers and 2,155 newborns were studied. The prevalence of infection by T. cruzi among these pregnant women is of 26,3%. Two groups of mothers were studied: 554 that presented infection by T. cruzi (group M+B-) and 1520 not infected (group control M-B-). Both groups of mothers are comparable in their anthropometric and obstetrical antecedents. The mothers (M+B+) are in average older than those not infected (p<0.05), which will probably have an influence on the number of gestations and abortion antecedents, which were of p<0.05 and p=0.01 respectively. Among the different anthropometric and biological parameters studied in newborns of groups M+B- and M-B -, no statistically significant differences between both groups were found. It can be inferred that the chronic maternal infection by T. cruzi seems to have no clinical influence, neither on the course of the pregnancy nor during birth, if a group of T. cruzi infected mothers is compared to a non infected group.
[Estimation of the parasitemia in Trypanosoma cruzi human infection: high parasitemias are associated with severe and fatal congenital Chagas disease].
(2005) Torrico, Mary Cruz; Solano, Marco; Guzmán, José Miguel; Parrado, Rudy; Suarez, Eduardo; Alonzo-Vega, Cristina; Truyens, Carine; Carlier, Yves; Torrico, Faustino
The aim of this study was to validate the method of microhematocrit tube, as a rapid method to estimate the parasitemia in blood and to associate the parasites concentration with the morbidity and mortality of new born children with congenital Chagas diseases. Our results were determined experimentally and shown that the detection limit of the microhematocrit tube method is 40 parasites/ml when at least one of the four observed tubes is positive. Besides, it was also established that when the four examined tubes are positive the parasitemia in blood reaches more than 100 parasites/ml. It is important to highlight the modification made by our laboratory in the microscopic observation of the microhematocrit tubes with respect to the methodology used by previous investigators. A positive association exists between a high number of parasites in blood and the morbi-mortality of the newly born children with congenital chagas. The results of positive association between the parasitic load and the morbility and mortality could constitute an argument to understand the possible role of the parasite in the pathology of the disease.