Browsing by Autor "Suzana Revollo"

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    In Vitro Benznidazole and Nifurtimox Susceptibility Profile of <em>Trypanosoma cruzi</em> Strains Belonging to Discrete Typing Units TcI, TcII and TcV
    (2019) Suzana Revollo; Bruno Oury; Andrea Vela; Michel Tibayrenc; Denis Séréno
    We ascertain the in vitro Benznidazole (BZN) and Nifurtimox (NFX) susceptibility pattern of epimastigotes, trypomastigotes, and amastigotes of 21 T. cruzi strains, from patients, reservoir and triatomine bugs of various geographic origin. Using this panel of isolates, we compute the Epidemiological cut off value (COwt). Then, the frequency of the susceptible phenotype (Wild type) towards BZN and nifurtimox (NFX) within this set of strains belonging to 3 discrete typing units (DTUs), TcI, TcII, and TcV was deduced. We have observed that the susceptibility status of individual T. cruzi isolates toward BZN and NFX is related to the genetic background and to underlying factors probably related to the individual life trait history of each strain. Analyzing drug susceptibility in this conceptual framework would offers the possibility to evidence a link between isolates expressing a low susceptibility level (not wild-type) as define by the COwt value and none-curative treatment. It will also permit to tract drug-resistant parasites in T. cruzi population.
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    Prevalence of Trypanosoma cruzi infection in blood banks of seven departments of Bolivia
    (Instituto Oswaldo Cruz, Ministério da Saúde, 1990) Roxana Carrasco; Hortensia Miguez; Clara Camacho; Lourdes Echalar; Suzana Revollo; Tania Ampuero; Jean-Pierre Dedet
    Trypanosoma cruzi infection was studied in 1,298 sera samples of blood banks from 7 capital departments of Bolivia, using the immunofluorescence test (IFI) and Enzyme Linked Immunosorbent Assay (ELISA). The percentages of positivity in these 7 departments have an average of 28% and are distributed as follows: Sta. Cruz 51%, Tarija 45%, Cochabamba 28%, Sucre 39%, La Paz 4.9%, Oruro 6% and Potosi 24%. The prevalence is related with the altitude levels of the different departments. However in Potosi (3,945 m) we found a 24% of prevalence, probably due to the proximity of endemic valleys to the city. The authors suggest a strict control in blood donors since there exists a great risk of infection.