Browsing by Autor "Thomas G. Ksiazek"

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    Chapare Virus, a Newly Discovered Arenavirus Isolated from a Fatal Hemorrhagic Fever Case in Bolivia
    (Public Library of Science, 2008) Simon Delgado; Bobbie R. Erickson; Roberto Agudo; Patrick J. Blair; Efraín Vallejo; César G. Albariño; Jorge Vargas; James A. Comer; Pierre E. Rollin; Thomas G. Ksiazek
    A small focus of hemorrhagic fever (HF) cases occurred near Cochabamba, Bolivia, in December 2003 and January 2004. Specimens were available from only one fatal case, which had a clinical course that included fever, headache, arthralgia, myalgia, and vomiting with subsequent deterioration and multiple hemorrhagic signs. A non-cytopathic virus was isolated from two of the patient serum samples, and identified as an arenavirus by IFA staining with a rabbit polyvalent antiserum raised against South American arenaviruses known to be associated with HF (Guanarito, Machupo, and Sabiá). RT-PCR analysis and subsequent analysis of the complete virus S and L RNA segment sequences identified the virus as a member of the New World Clade B arenaviruses, which includes all the pathogenic South American arenaviruses. The virus was shown to be most closely related to Sabiá virus, but with 26% and 30% nucleotide difference in the S and L segments, and 26%, 28%, 15% and 22% amino acid differences for the L, Z, N, and GP proteins, respectively, indicating the virus represents a newly discovered arenavirus, for which we propose the name Chapare virus. In conclusion, two different arenaviruses, Machupo and Chapare, can be associated with severe HF cases in Bolivia.
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    Reemergence of Bolivian Hemorrhagic Fever, 2007–2008
    (Centers for Disease Control and Prevention, 2009) Patricia V. Aguilar; Wilfredo Camargo; Jorge Vargas; Carolina Guevara; Yelin Roca; Vidal Felices; V. Alberto Laguna-Torres; Robert B. Tesh; Thomas G. Ksiazek; Tadeusz J. Kochel
    age.In particular, suspected childhood TB patients without an identifiable TB contact and with normal immune status were subjected to further investigations.Multidisciplinary management, including enhanced laboratory diagnosis of atypical bony lesions in infants and children, is recommended for any suspected TB infection.Once BCGrelated infection is confirmed, medical treatment has to be consistent.