Browsing by Autor "Torrico Villarroel, Nattaly Grecia"

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    Alternativas de diagnóstico de laboratorio para la detección del virus de la Influenza
    (Gac Med Bol, 2019) Angulo Valdivia, Cecilia; Torrico Villarroel, Nattaly Grecia
    El virus de la Influenza (Flu) ha sido causante de diversas pandemias que han producido millones de muertes a nivel mundial, siendo necesario un diagnóstico diferencial al producir un cuadro clínico similar al de infecciones producidas por otros virus respiratorios. El diagnóstico oportuno y certero de infección por Influenza virus empleando pruebas de laboratorio, permite una mejor toma de decisiones en el ámbito médico, de tal manera que se pueda administrar un tratamiento adecuado, disminuir complicaciones y costos de hospitalización. A pesar de existir diferentes alternativas de métodos de diagnóstico, desde técnicas tradicionales como el cultivo viral o la inmunofluorescencia, hasta técnicas de detección de ácidos nucleicos, todas presentan ventajas y desventajas en cuanto al tiempo de procesamiento y entrega de resultados, sensibilidad, especificidad, experticia del personal, costos e infraestructura del laboratorio, siendo estos factores importantes a considerar para la implementación de nuevas técnicas en los laboratorios clínicos.
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    Multiplex RT-qPCR strategy for SARS-CoV-2 variants detection in developing countries without ngs: The Bolivian experience.
    (2025) Parrado, Rudy; Cuba-Grandy, Carolina X; Fuentes-Luppichini, Eugenia; Torrico Villarroel, Nattaly Grecia; Mamani-Ortiz, Yercin; Mendez, Jaqueline; Melgarejo, Betty; Coronado-Arrázola, Irenice; Montaño, Nair A; Almonacid, Leonardo I; Medina, Rafael A; Garcia, Lineth; Pardo-Roa, Catalina
    The rapid evolution of SARS-CoV-2 has led to the emergence of variants of concern (VOCs) characterized by increased transmissibility, pathogenicity, and resistance to neutralizing antibodies. Identifying these variants is essential for guiding public health efforts to control COVID-19. Although whole genome sequencing (WGS) is the gold standard for variant identification, its implementation is often limited in developing countries due to resource constraints. In Bolivia, genomic surveillance is a challenge due to its limited technological infrastructure and resources. An RT-qPCR-based strategy was designed to address these limitations and detect the mutations associated with VOCs and variants of interest (VOIs). The multiplex RT-qPCR commercial kits AllplexTM Master and Variants I (Seegene®) and the ValuPanelTM (Biosearch®) were used to target mutations such as HV69/70del, E484K, N501Y, P681H, and K417N/T. They are characteristic of the Alpha (B.1.1.7), Beta (B.1.531), Gamma (P.1), Omicron (B.1.1.529), Mu (B.1.621), and Zeta (P.2) variants. A total of 157 samples collected in Cochabamba from January to November 2021 were evaluated, identifying 44 Gamma, 2 Zeta, 20 Mu, and 10 Omicron were identified. The strategy's effectiveness was validated against WGS data generated with Oxford NanoporeTM technology, showing a concordance rate of 0.96. This highlights the value of the RT-qPCR strategy in guiding the selection of samples for WGS, enabling broader detection of new variants that cannot be identified by RT-qPCR alone.