Browsing by Autor "Valdir Cechinel Filho"

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Antiparasitic activity of two Brazilian plants: Eugenia mattosii and Marlierea eugeniopsoides
(Taylor & Francis, 2020) Giovana Vechi; Adrielli Tenfen; Efraín Salamanca; Alberto Giménez; Valdir Cechinel Filho
Parasitoses are very common throughout the world, generating serious consequences for public health. Leishmaniosis and giardiasis are examples of fairly recurrent, but neglected diseases. Several higher plants have demonstrated promising activity against the parasites. The aim of this study was to evaluate the biological activity of extracts, fractions and isolated compounds from the leaves and stems of two Brazilian plants: Eugenia mattosii and Marlierea eugeniopsoides (Myrtaceae) against Leishmania and Giardia. XTT and the fluorimetric method were used to for this evaluation, respectively. Cytotoxicity was evaluated against HeLa cells. The results demonstrated that chloroform fractions of E. matosii and pinostrobin presented the most pronounced antiparasitic activity, with the CLF-stems being the most effective against Leishmania amazonensis and Leishmania braziliensis. Pinostrobin also presented activity against G. lamblia. Therefore, E. mattosii stems and pinostrobin may be considered possible targets for the continuity of studies against other parasites.
Drimanes from Drimys brasiliensis with leishmanicidal and antimalarial activity
(Instituto Oswaldo Cruz, Ministério da Saúde, 2013) Vanessa D. Claudino; Kesia Caroline da Silva; Valdir Cechinel Filho; Rosendo A. Yunes; Franco Delle Monachè; Alberto Giménez; Efraín Salamanca; David Gutierrez-Yapu; Ângela Malheiros
This paper evaluates CHCl3 and CH3OH extracts of the stem bark, branches and leaves of Drimys brasiliensis and drimane sesquiterpenes isolated from the stem bark against strains of Leishmania amazonensis and Leishmania braziliensis promastigotes and Plasmodium falciparum trophozoites. All of the extracts and compounds were tested in cell lines in comparison with reference standards and cell viability was determined by the XTT method. The CHCl3 and CH3OH extracts from the stem bark and branches yielded promising results against two strains of Leishmania, with 50% inhibitory concentrations (IC50 ) values ranging from 39-100 µg/mL. The CHCl3 extract of the stem bark returned IC50 values of 39 and 40.6 µg/mL for L. amazonensis and L. braziliensis, respectively. The drimanes were relatively effective: 1-β-(p-coumaroyloxy)-polygodial produced IC50 values of 5.55 and 2.52 µM for L. amazonensis and L. braziliensis, respectively, compared with 1-β-(p-methoxycinnamoyl)-polygodial, which produced respective IC50 values of 15.85 and 17.80 µM. The CHCl3 extract demonstrated activity (IC50 of 3.0 µg/mL) against P. falciparum. The IC50 values of 1-β-(p-cumaroyloxyl)-polygodial and 1-β-(p-methoxycinnamoyl)-polygodial were 1.01 and 4.87 µM, respectively, for the trophozoite strain. Therefore, the results suggest that D. brasiliensis is a promising plant from which to obtain new and effective antiparasitic agents.
Evaluation of Antileishmanial Activity of Selected Brazilian Plants and Identification of the Active Principles
(Hindawi Publishing Corporation, 2013) Valdir Cechinel Filho; Christiane Meyre‐Silva; Rivaldo Niero; Luísa Nathália Bolda Mariano; Fabiana Gomes do Nascimento; Ingrid Vicente Farias; Vanessa Fátima Gazoni; Bruna dos Santos Silva; Alberto Giménez; David Gutierrez-Yapu
This study evaluated extracts, fractions, and isolated compounds from some selected Brazilian medicinal plants against strains of promastigotes of Leishmania amazonensis and L. brasiliensis in vitro. The cell viability was determined, comparing the results with reference standards. The dichloromethane fractions of the roots, stems, and leaves of Allamanda schottii showed IC50 values between 14.0 and 2.0 μ g/mL. Plumericin was the main active compound, with IC50 of 0.3 and 0.04 μ g/mL against the two species of Leishmania analyzed. The hexane extract of Eugenia umbelliflora fruits showed IC50 of 14.3 and 5.7 μ g/mL against L. amazonensis and L. brasiliensis, respectively. The methanolic extracts of the seeds of Garcinia achachairu and guttiferone A presented IC50 values of 35.9 and 10.4 μ g/mL, against L. amazonensis, respectively. The ethanolic extracts of the stem barks of Rapanea ferruginea and the isolated compound, myrsinoic acid B, presented activity against L. brasiliensis with IC50 of 24.1 and 6.1 μ g/mL. Chloroform fraction of Solanum sisymbriifolium exhibited IC50 of 33.8 and 20.5 μ g/mL, and cilistol A was the main active principle, with IC50 of 6.6 and 3.1 μ g/mL against L. amazonensis and L. brasiliensis, respectively. It is concluded that the analyzed plants are promising as new and effective antiparasitic agents.
Gastroprotective activity of alkaloid extract and 2-phenylquinoline obtained from the bark of Galipea longiflora Krause (Rutaceae)
(Elsevier BV, 2009) Francielle Zanatta; Renan Becker Gandolfi; Marivane Lemos; Juan C. Ticona; Alberto Giménez; Bruna Kurz Clasen; Valdir Cechinel Filho; Sérgio Faloni de Andrade
Gastroprotective and anti-secretory mechanisms of 2-phenylquinoline, an alkaloid isolated from Galipea longiflora
(Elsevier BV, 2016) Eduardo Breviglieri; Luísa Mota da Silva; Thaise Boeing; Líncon Bordignon Somensi; Benhur Judah Cury; Alberto Giménez; Valdir Cechinel Filho; Sérgio Faloni de Andrade
Preparations from Campomanesia reitziana reduce the gastrointestinal motility and castor oil‐induced diarrhea in a non‐opioid and non‐dopaminergic pathway in mice and display antimicrobial activity in vitro
(Wiley, 2021) Marihá Mariott; Luísa Nathália Bolda Mariano; Thaise Boeing; Camile Cecconi Cechinel‐Zanchett; Efraín Salamanca; Alexandre Bella Cruz; Alberto Giménez; Valdir Cechinel Filho; Luísa Mota da Silva
The AECR and EAF can be effective to treat diarrhea acting through opioid- or dopaminergic type 2 receptor-independent mechanisms and by its antimicrobial actions.
Value of the ethnomedical information for the discovery of plants with antifungal properties. A survey among seven Latin American countries
(Elsevier BV, 2009) Laura Svetaz; Federico Zuljan; Marcos Derita; Elisa M. Petenatti; Giselle Tamayo‐Castillo; Armando Cáceres; Valdir Cechinel Filho; Alberto Giménez; Roberto Pinzón; Susana Zacchino