Browsing by Autor "Vargas, Virginia A"

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LIPOLYTIC ENZYME PRODUCTION BY HALOPHILIC/HALOTOLERANT MICROORGANISMS ISOLATED FROM LAGUNA VERDE, BOLIVIA
(Rev. Bol. Quim, 2008) Niño de Guzmán, Mariana; Vargas, Virginia A; Antezana, Henry; Svoboda, Michal
Bacterial strains isolated from water samples from Laguna Verde, Potosi-Bolivia and deposited at Biotechnology Center, Cochabamba-Bolivia, were subjected to lipolytic enzyme production studies. Among these, 10 strains were selected as positive in lipolytic enzyme production in solid medium, using olive oil as sole carbon source. Selected strains were subjected to morphological and biochemical studies. Enzyme production in liquid medium identified strain LV01 as the best enzyme producer with 0.079 U/ml lipolytic activity. Partial purification by ion-exchange chromatography of the lipolytic enzyme, produced by strain LV01, presented specific enzyme activity of 0,138 U/mg of eluted protein with approximately 77 KDa size. Partial characterization of the produced enzyme revealed its display maximum activity values at pH 9, 30°C y 1.7M NaCl , thus exhibiting remarkably haloalkalitolerant properties. The 16S rDNA gene sequence analysis showed 99% similarity between strain LV01 and Bacillus pumilus (embAJ494727)
XYLANASE PRODUCTION USING BARLEY STRAW BY BACILLUS SP. LB-4 ISOLATED FROM LAGUNA BLANCA, POTOSI-BOLIVIA
(Rev. Bol. Quim, 2012) Gandarillas, Cecilia; Soto, Roberto; Vargas, Virginia A
Estudio sobre la producción de enzimas xilanolíticas en bacterias colectadas en Laguna Blanca, Potosi-Bolivia. La caracterización parcial de las enzimas producidas que mostraron una máxima actividad a un pH 9 a una concentración de NaCl de 50°C y 0% (w/v) exhibiendo así propiedades termo-alkalitolerantes. El análisis de la secuencia del gen 16S rDNA mostró un 99% de similaridad entre la cepa LB-4 y Bacillus pumilus (embAJ494727).