Browsing by Autor "Vino-Nina Nicolasa Lourdes"

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Biocontrol capacity of Beauveria brongniartii (Sacc.) and Metarhizium anisopliae (Metsch.) in the control of aphids Macrosiphum euphorbiae (Hemiptera: Aphididae)
(2017) Rojas-Gutiérrez René Leopoldo; Loza-Murguia Manuel Gregorio; Vino-Nina Nicolasa Lourdes; Teófilo Serrano-Canaviri
Aiming to develop alternative technologies in controlling aphids (Macrosiphum euporviae), the biocontrol ability of entomopathogenic fungi Beauveria brongniartii and Metarhizium anisopliae evaluated. Par study native fungi of 3 parts of the central highlands (Achacachi , Viacha and Patacamaya) , which is isolated from the mummified insects and purified in the laboratory, the isolates were as fungi of the genus Beauveria result and Metarhizium were collected. Of these isolates its initial concentration was removed and all were adjusted to the minimum concentration lodged in one of the isolates was 9.5 x 10⁴conidia/mL at these concentrations the virulence test was performed for 144 hours , where isolated 2BbVch , 1BbAch , 3BbPtm 1MtAch and produced more than 50 % mortality , indicating that the genus Beauveria isolates proved to be better . With isolates that demonstrated improved mortality were subjected to a second test determining the lethal time 50 and 80 ( LT50 ) ( TL80 ) where 2BbVch isolated showed a lower time control 50 and 80% with 78 aphids 1 and 123.8 hours respectively . 50 and the lethal concentration 80 ( IC50 ) was determined, (IC80), where it was observed that the isolated 1BbAch 2BbVch and require minimal concentrations of 2.5 × 10⁴ and 5.1 x 10⁴ conidia/mL, to control 50% of aphids as a concentration of 1.3 x 10⁵ to 1.8 x10⁵ conidia/mL for 80 % control of aphids.
Effect of six energetic substrates on the time of sperm viability of the semen of Alpaca (Vicugna pacos L.)
(2017) Paxipatty-Quispe Víctor Abdón; Loza-Murguia Manuel Gregorio; Vino-Nina Nicolasa Lourdes; Felipe Chura-Limachi; Gonzales-Vargas Víctor Efraín; Delgado-Callisaya Pedro Ángel
The research was carried out in the Laboratory of Reproductive Biotechnology of the UAC Tiahuanacu, Ingavi province of the Department of La Paz, dependent of the Bolivian Catholic University, whose objective was to evaluate the effect of six energetic substrates on the sperm viability time of the alpaca semen (Vicugna pacos L.). The collected semen was diluted and stored in six different energy substrates (TCG, TCAP, TCF, TG, TAP and TF), a T ° of 35 ºC, evaluating every 2 hours, using the Completely Random Design (P < 0.05). Obtaining a mean volume of semen 1.5 ± 0.7 mL, filament 5.1 ± 1.5, pH 7.2 ± 0.5, motility and vitality of 89.0 and 95.0% and concentration 82.0x106 Epz / mL. The energetic substrate (SE) TCG managed to maintain a motility of 61.0 and 66.0% of vitality preserved for 8 hours, in that sense it is recommended to use the SE TCG, which maintained for a longer time (hours) the viable spermatozoa for LAI.
Filamentous soil fungi comunities on Kiphakiphani agroesosistems, Choquenaira-Viacha Municipaly
(2017) Pacasa-Quisbert Fernando; Loza-Murguia Manuel Gregorio; Bonifacio-Flores Alejandro; Vino-Nina Nicolasa Lourdes; Teófilo Serrano-Canaviri
Soil fungi play important roles in ecosystems and these populations are closely related to soil type, climatic characteristics, other microorganisms and plant communities. People’s activities, mainly agriculture alter their habitat and therefore affect fungi diversity and functions on soil. The effects of soil use type (TUS), native vegetation type (TVN) and crop type (TC) on colony forming units (CFU), number of spe-cies and physical, chemical and biological parameters was assessments in the present work in Plurination-al State of Bolivian´s highland agroecosystem. In addition, the diversity and number of filamentous fungi were related to physical and chemical soil parameters (pH, texture, organic matter), The fungi were de-scribed macro- and microscopically and were taxonomically identified. The TUS, TVN and TC influenced differently on the pH, soil texture and soil organic matter (SOM). The TUS and TC significantly affected the CFU´s and number of species. CFU´s were high in crop potato and number of species in Trifolium sp., 44 species were isolated, predominating genera of Penicillium and Alternaria. The rela-tionships between SOM were non-significant and weak (r2 = 32.1, p> 0.05), pH, significant and strong positive relation (r2 = 47.7, p <0.05) and soil texture vs CFU´s no significant and unrelated (r2 = 8.6, p> 0.05). The results suggest that the soil use type affect in soil properties, plant diversity and community fungi. Additionally, it is necessary to investigate other parameters that influence the microbial community in the agroecosystem.
Oestrus ovis (Diptera: Oestridae) an important ectoparasite in sheep of four cantons of the municipality of Sorata province Larecaja, department of La Paz
(2017) Choque-Fernández Graciela Cristina; Loza-Murguia Manuel Gregorio; Vino-Nina Nicolasa Lourdes; Coria-Conde Luis Alfredo
The study of Oestrosis in sheep was carried out in the cantons of Sorata, Obispo Bosque, Laripata and Ilabaya, belonging to the Municipality of Sorata, under the agreement made with the institution Departmental Agricultural and Livestock Service (SEDAG) between August and September 2002, with 164 heads of sheep out of which 151 heads were Oestrus ovis, 13 were negative, taking into account the factors canton, sex, age and number of larvae per animal. The prevalence of Oestrosis in Sorata was 39% and by corner Obispo Bosque 28%, Ilabaya and Laripata 18% and 15% respectively. The analysis of variance to quantify the number of larvae per canton did not present significant differences (P> 0.005) as well as for the sex and age factor, which means that the canton Sorata, Obispo Bosque, Laripata and Ilabaya have equal % of Oestrosis for The age factor is not significant difference between females and males nor groups of age 1 and 2. Analysis of variance to determine the differences in the number of larvae per canton, sex, age and larval stage also does not exist significant differences, however, considering (P <0.005) because there are more larvae in the L-1 stage than L-2 and L-3. In this sense, Oestrosis affects all the cantons under study without distinction of age groups, sex, however varying in the presence of different larval stages, where L-1 predominates.