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Browsing by Autor "Yvonne Granfeldt"

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    Effect of fermentation and dry roasting on the nutritional quality and sensory attributes of quinoa
    (Wiley, 2019) Vanesa Castro‐Alba; Claudia E. Lazarte; Daysi Perez‐Rea; Ann‐Sofie Sandberg; Nils‐Gunnar Carlsson; Annette Almgren; Björn Bergenståhl; Yvonne Granfeldt
    Fermentation of quinoa flour for 4 hr followed by dry roasting was successful in improving both nutritional and sensory attributes of the final product.
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    Fermentation of pseudocereals quinoa, canihua, and amaranth to improve mineral accessibility through degradation of phytate
    (Wiley, 2019) Vanesa Castro‐Alba; Claudia E. Lazarte; Daysi Perez‐Rea; Nils‐Gunnar Carlsson; Annette Almgren; Björn Bergenståhl; Yvonne Granfeldt
    Fermentation proved to be effective for degrading phytate in pseudocereal flours, but less so in grains. Fermentation with Lactobacillus plantarum 299v® improved mineral accessibility and estimated bioavailability in flours. © 2019 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
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    Fermented Quinoa and Canihua in Plant‐Based Diets Increase Iron and Zinc Bioavailability in Growing Rats
    (Wiley, 2024) Vanesa Castro‐Alba; Mirian Vargas; Ann‐Sofie Sandberg; Daysi Perez‐Rea; Björn Bergenståhl; Yvonne Granfeldt; Claudia E. Lazarte
    This study aimed at evaluating the effects of non-fermented and fermented pseudocereal flours, quinoa and canihua, on iron and zinc bioavailability in Wistar rats. Two diets prepared with 92% fermented quinoa or 79.5% fermented canihua were compared with diets prepared with the same amount of non-fermented pseudocereals. Other two quinoa diets were prepared with 60% non-fermented or fermented quinoa and compared with a refence diet which was free of phytates. Body weight, feed efficiency ratio, and the absorption, retention and bioavailability of iron and zinc were evaluated. While body weight and feed efficiency ratio were higher (<i>p</i> < 0.05) in animals after non-fermented diets, the results of mineral absorption and bioavailability were consistently higher in the diets containing fermented pseudocereals. Iron concentration in the livers of animals after the fermented quinoa (92%) and canihua diet (79.5%), were 34% and 30% higher than after the diets with non-fermented pseudocereals. Zinc bioavailability, indicated by zinc in femur of animals fed the 60% fermented quinoa diet was 53.2 μg g<sup>-1</sup> Zn<sub>Int</sub> g<sup>-1</sup> BW, comparable to that in animals fed a reference diet with no phytates (58.2 μg g<sup>-1</sup> Zn<sub>Int</sub> g<sup>-1</sup> BW), and significantly higher (<i>p</i> < 0.05) than in animals fed the non-fermented quinoa diet (34.5 μg g<sup>-1</sup> Zn<sub>Int</sub> g<sup>-1</sup> BW). Zinc bioavailability was mainly influenced by phytate content in the diet (<i>R</i> <sup>2</sup> = 0.665 and <i>p</i> = 0.000). The retention of iron in the liver (2220 μg g<sup>-1</sup> Fe<sub>Int</sub> g<sup>-1</sup> BW) was higher in the diet containing 60% of fermented quinoa than in the non-fermented diet (1429 μg g<sup>-1</sup> Zn<sub>Int</sub> g<sup>-1</sup> BW). Differences in iron absorption were mainly impacted by iron content in the diets (<i>R</i> <sup>2</sup> = 0.828 and <i>p</i> = 0.000). In conclusion, the addition of fermented pseudocereals to diets increased the bioavailability of iron and zinc in Wistar rats. These findings will encourage further research into fermented pseudocereals and their potential health effects.
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    Nutritional Status of Children with Intestinal Parasites from a Tropical Area of Bolivia, Emphasis on Zinc and Iron Status
    (Scientific Research Publishing, 2015) Claudia E. Lazarte; Andrea Brenes Soto; Leovegildo Alvarez; Björn Bergenståhl; Nora Medrano; Yvonne Granfeldt
    Malnutrition and parasitic diseases are within the major issues in rural areas in developing countries. In this study, the nutritional status, dietary intake including mineral absorption inhibitor (phytate), hematological indicators and trace element status (zinc, iron) were evaluated and associated to the presence of intestinal parasites in a group of children from a rural area of Bolivia. The results showed that 96% of the children had intestinal parasites; 7 types of parasites (Ascaris lumbricoides, Giardia lamblia, Ancylostoma duodenale, Entamoeba histolytica, Entamoeba coli, Trichuris trichiura, Strongyloides stercolaris) were identified. Anthropometric measurements indicated that 37% of the children were stunted and 17% were underweight. Iron and zinc intake showed that 34% and 30% of children had inadequate intake of these nutrients respectively. Phytate: zinc molar ratios were between 6.5 and 21, and from 6.2 to 15 for phytate: iron, indicating that the absorption of zinc and iron might be compromised by the level of phytate in the diet. The serum zinc was below the lower cut-off in 87% of the children, indicating zinc deficiency. Moreover, a multiple regression model showed the significant effect of the presence of the parasite Giardia lamblia and phytate intake on the serum zinc levels. Regarding the iron status, 30% of the children presented with anemia and about 66% had iron deficiency; a simple linear regression model showed the significant negative effect of the presence of the parasite Ancylostoma duodenale on iron status. In conclusion, the levels of zinc and iron, which were low in this child population, were greatly affected by the presence of intestinal parasites; in addition, the consumption of plant-based diets with high levels of phytate also impaired the zinc absorption.
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    Nutritional Status of Patients with Cutaneous Leishmaniasis from a Tropical Area of Bolivia, and Implications for Zinc Bioavailability
    (Scientific Research Publishing, 2013) Claudia E. Lazarte; Claudia Alegre; Ernesto Rojas; Yvonne Granfeldt
    Macro and micronutrient deficiencies are a significant problem among people in rural areas in developing countries. Deficiencies may lead to an impaired immune system making the organism vulnerable to infections and diseases. In this paper, the dietary intake, anthropometric measurements, zinc and copper levels in serum, of patients with cutaneous leishmaniasis (CL) are compared with a group of healthy controls and reference values. Results showed no significant differences in most nutrient intake or anthropometrics between patients and controls. However, serum zinc level of patients (80 g/dl) was significantly lower (P < 0.001) than in controls (85 g/dl), likely explained by the presence of leishmaniasis. The median serum zinc level in both groups was below the reference values, even though their median zinc intake met the zinc recommendations from WHO. Consequently, besides the presence of leishmaniasis, serum zinc levels may be compromised by inhibitory components in their diet, such as phytates, as it is shown by the molar ratio phytate:zinc (Phy:Zn) that was between 11 and 19, while 15 is the level said to compromise zinc status. There were significant (P < 0.05) negative correlations between serum zinc and Phy:Zn, for patients (r = -0.413) and controls (r = -0.410). In conclusion this study shows that patients with CL in Chapare, Bolivia had low serum zinc levels due to the leishmaniasis per se and the decreased zinc bioavailability in their diet. CL infection was not in direct association with the nutritional status indicated by the anthropometric or dietary assessments. However, dietary intake showed 5 essential nutrients below the nutrient recommendation in both groups.
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    Phytate, iron, zinc, and calcium content of common Bolivian foods and their estimated mineral bioavailability
    (Wiley, 2019) Vanesa Castro‐Alba; Claudia E. Lazarte; Björn Bergenståhl; Yvonne Granfeldt
    There is a scarcity of information on mineral and phytate content in plant-based foods in Bolivia. This study aimed to analyze iron, zinc, calcium, and phytate content and estimate the mineral bioavailability of foods consumed in Chapare, Bolivia. Minerals and phytate were analyzed, and bioavailability was estimated in 17 food samples. Leafy vegetables and green legumes had the highest mineral content, followed by pseudocereals. Estimated mineral bioavailability was low for cereals, dry legumes, pseudocereals, and flaxseeds foods mainly due to phytate content. But estimated zinc bioavailability for black cornmeal, yellow corn, and dry peas was moderate. Strong correlations (<i>p</i> < 0.01) were found between the three minerals, while phytate correlated negatively to iron, zinc, and calcium. To get an overview of the estimated mineral bioavailability of plant-based diets, we have included foods, from the same area, analyzed in a previous study where the evaluated diet covers 80% of RNI for iron and zinc, but <40% of calcium. In conclusion, leafy vegetables and green legumes had the highest contents of minerals and the lowest phytate content of the foods analyzed in the study. The usage of processing strategies and dietary diversification to reduce phytate content would significantly improve estimated mineral bioavailability in plant-based diets.
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    Phytate, zinc, iron and calcium content of common Bolivian food, and implications for mineral bioavailability
    (Elsevier BV, 2015) Claudia E. Lazarte; Nils‐Gunnar Carlsson; Annette Almgren; Ann‐Sofie Sandberg; Yvonne Granfeldt
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    Quinoa Fermentation and Dry Roasting to Improve Nutritional Quality and Sensory Properties
    (2021) Claudia E. Lazarte; Vanesa Castro‐Alba; Yvonne Granfeldt
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    Validation of digital photographs, as a tool in 24-h recall, for the improvement of dietary assessment among rural populations in developing countries
    (BioMed Central, 2012) Claudia E. Lazarte; Ma Eugenia Encinas; Claudia Alegre; Yvonne Granfeldt
    The FP 24-hR exhibits acceptable differences when compared with a WFR, digital photos are useful as a memory aid for the subjects during 24-h recall and as an estimation tool. The method is suitable for assessing dietary intake among rural populations in developing countries.
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    Zinc bioavailability in rats fed a plant-based diet: a study of fermentation and zinc supplementation
    (Swedish Nutrition Foundation, 2015) Claudia E. Lazarte; Mirian Vargas; Yvonne Granfeldt
    In conclusion, the fermentation of cassava reduces the phytate content. The diet containing the fermented cassava represents a better nutritional alternative than the diet with unfermented cassava and is comparable with the zinc-supplemented diets.

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