Browsing by Autor "Zulema Bustamante"

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Acinetobacter baumannii analysis by core genome multi-locus sequence typing in two hospitals in Bolivia: endemicity of international clone 7 isolates (CC25)
(Elsevier BV, 2019) Mónica Cerezales; Kyriaki Xanthopoulou; Julia Wille; Zulema Bustamante; Harald Seifert; Lucía Gallego; Paul G. Higgins
CONGENITAL CHAGAS DISEASE IN BOLIVIA IS NOT ASSOCIATED WITH DNA POLYMORPHISM OF TRYPANOSOMA CRUZI
(American Society of Tropical Medicine and Hygiene, 2006) Myrna Virreira; Cristina Alonso‐Vega; Marco Solano; Juan Jijena; Laurent Brutus; Zulema Bustamante; Carine Truyens; Dominique Schneider; Faustino Torrico; Yves Carlier
This study aims to typify the Trypanosoma cruzi (sub)lineage(s) in umbilical cord blood of congenitally infected Bolivian newborns, using PCR amplifications of "Region Markers", mini-exon or kDNA fragments followed by hybridization or sequencing. New probes were also designed to distinguish three variants within the TcIId sublineage. The IIb, IId, or IIe T. cruzi sublineages, as well as different variants of the IId sublineage, were detected in infected neonates, whereas mixed infections were not found. The frequencies of the IId sublineage were similar in neonates (95.1%) and adults of the same area (94.1%). The IId-infected newborns displayed either asymptomatic, or severe and fatal clinical forms of congenital Chagas disease, as well as low or high parasitemia. Altogether these data show that T. cruzi DNA polymorphism, based on the presently available markers, is not associated with the occurrence of congenital infection or the development of severe clinical forms of congenital Chagas disease.
Diagnóstico y Percepción de los Jóvenes Universitarios y las Autoridades de Seguridad Ciudadana de la Ciudad de Oruro
(Latin American Association for the Advancement of Sciences, 2023) Elisabet Calani Carrión; Zulema Bustamante
En Bolivia, en los últimos años el número de casos de inseguridad ciudadana han aumentado notablemente, dentro de ella una comunicación deficiente influye en la confianza de los jóvenes universitarios hacia las autoridades de seguridad ciudadana. Este artículo presenta las principales causas de desconfianza hacia esta institución y cómo es que la comunicación influye en ella, por otro lado, se da conocer las estrategias comunicacionales que aplican las autoridades de seguridad ciudadana, con datos certeros. A través de este estudio, damos a conocer la importancia de la comunicación en ámbitos de la seguridad ciudadana, siendo que es un tema sumamente importante en nuestro cotidiano vivir, resaltando que nuestras relaciones humanas son efectivas cuando la comunicación es empleada de manera correcta, teniendo en cuenta que tal es indispensable en todos los ámbitos de nuestra vida.
Emergence and clonal dissemination of carbapenem-hydrolysing OXA-58-producing Acinetobacter baumannii isolates in Bolivia
(Microbiology Society, 2011) Elena Sevillano; Elena Fernández; Zulema Bustamante; Silvia Zabalaga; Ikerne Rosales; A. Umaran; Lucía Gallego
Acinetobacter baumannii is an emerging multidrug-resistant pathogen and very little information is available regarding its imipenem resistance in Latin American countries such as Bolivia. This study investigated the antimicrobial resistance profile of 46 clinical strains from different hospitals in Cochabamba, Bolivia, from March 2008 to July 2009, and the presence of carbapenemases as a mechanism of resistance to imipenem. Isolates were obtained from 46 patients (one isolate per patient; 30 males,16 females) with an age range of 1 day to 84 years, and were collected from different sample types, the majority from respiratory tract infections (17) and wounds (13). Resistance to imipenem was detected in 15 isolates collected from different hospitals of the city. These isolates grouped into the same genotype, named A, and were resistant to all antibiotics tested including imipenem, with susceptibility only to colistin. Experiments to detect carbapenemases revealed the presence of the OXA-58 carbapenemase. Further analysis revealed the location of the bla(OXA-58) gene on a 40 kb plasmid. To our knowledge, this is the first report of carbapenem resistance in A. baumannii isolates from Bolivia that is conferred by the OXA-58 carbapenemase. The presence of this gene in a multidrug-resistant clone and its location within a plasmid is of great concern with regard to the spread of carbapenem-resistant A. baumannii in the hospital environment in Bolivia.
First detection of the blaOXA-23 gene in a multidrug-resistant A. baumannii clinical isolate from Cochabamba, Bolivia
(Open Learning on Enteric Pathogens, 2016) Elena Sevillano; Mirella Moreno; Fatima Funes; Magaly Espinoza; Zulema Bustamante; Lucía Gallego
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High Prevalence of Extensively Drug-resistant Acinetobacter baumannii at a Children Hospital in Bolivia
(Lippincott Williams & Wilkins, 2018) Mónica Cerezales; Alain A. Ocampo-Sosa; Laura Álvarez Montes; Catalina Díaz Ríos; Zulema Bustamante; Jazmín Santos; Luis Martínez‐Martínez; Paul G. Higgins; Lucía Gallego
Acinetobacter baumannii causes serious hospital-acquired infections and has been positioned as a priority organism by the World Health Organization. This study includes 36 A. baumannii isolates from a children hospital recovered between March 2014 and May 2015 in Cochabamba. The majority of the isolates were recovered from blood cultures (n = 10, 31.3%) and respiratory samples (n = 11, 34.4%); 53% of the patients were younger than 1 month old. Most of these isolates (n = 30, 80.6%) were extremely drug resistant and 8.3% were multidrug resistant. The circulation of 2 predominant clones including 25 isolates was determined by pulsed-field gel electrophoresis; 9 of the isolates were considered sporadic strains. The isolates grouped in the predominant clones and 5 of the unrelated sporadic strains were single-locus variant or double locus variant of clonal complex (CC110), belonging to international clone 7; the rest of the isolates were single-locus variant or double locus variant of another clonal complex. All the carbapenem-resistant isolates (88.9%) carried the blaOXA-23-like in a similar structure to Tn2008 located on the chromosome, and the aac(3)-IIa gene was present in all the aminoglycoside-resistant isolates (86.1%). Strong biofilm producers were found among these isolates, being the strongest ones those recovered from the hospital environment, catheter, blood and cerebrospinal fluid (CSF) all of them belonged to the unrelated sporadic strains. The present study demonstrated the predominance and spread of closely related extremely drug-resistant A. baumannii isolates, what confers increasing risk to children and is of major concern because of the kind of infections and the lack of therapeutic alternatives to treat them.
Identification of Acinetobacter seifertii isolated from Bolivian hospitals
(Microbiology Society, 2018) Mónica Cerezales; Kyriaki Xanthopoulou; Julia Ertel; Alexandr Nemec; Zulema Bustamante; Harald Seifert; Lucía Gallego; Paul G. Higgins
Acinetobacter seifertii is a recently described species that belongs to the Acinetobacter calcoaceticus-Acinetobacter baumannii complex. It has been recovered from clinical samples and is sometimes associated with antimicrobial resistance determinants. We present here the case of three A. seifertii clinical isolates which were initially identified as Acinetobacter sp. by phenotypic methods but no identification at the species level was achieved using semi-automated identification methods. The isolates were further analysed by whole genome sequencing and identified as A. seifertii. Due to the fact that A. seifertii has been isolated from serious infections such as respiratory tract and bloodstream infections, we emphasize the importance of correctly identifying isolates of the genus Acinetobacter at the species level to gain a deeper knowledge of their prevalence and clinical impact.