Alexis St‐GelaisEliana M. MaldonadoGloria SaavedraSamuel Siles-AlvaradoJérôme AlsarrafGuy J. CollinAndré Pichette2026-03-222026-03-22202110.3390/molecules26195766https://doi.org/10.3390/molecules26195766https://andeanlibrary.org/handle/123456789/52350Citaciones: 4Over 15 years, with the support of a Canadian funding agency, the Universidad Mayor de San Simón, in Bolivia, undertook a large survey of aromatic plants of the South American country. More than a hundred species were studied under various aspects, including the production and characterization of essential oils. As part of this survey, the chemical composition of an essential oil sample obtained from <i>Pentacalia herzogii</i> (Asteraceae) growing wild in the High Valley region of the department of Cochabamba was determined by a combination of GC and GC-MS measurements. α-Pinene was the main constituent of this essential oil (34%), accompanied by limonene (22%) and germacrene D (7.5%) as well as an important fraction of methoxylated monoterpenoids. They were mainly isomers of thymol methyl ether, accounting for 13% of the chromatogram. A new quantitatively important compound (9%) was identified through NMR and chemical synthesis as 4-isopropyl-6-methylbenzo[<i>d</i>][1,3]dioxole, and designated herzogole, alongside the minor related compound 1-isopropyl-2,3-dimethoxy-5-methylbenzene. The monoterpene benzodioxole featured a distinctive green-phenolic aroma which could raise interest for fragrance use. Since these compounds were not known naturally, a biosynthetic mechanism of their formation was proposed and put in perspective to illustrate the metabolic originality of <i>P. herzogii</i>.enMonoterpeneEssential oilChemistryLimoneneEtherIsopropylGermacreneAsteraceaeTerpeneBotanyarticle