Tomás DuffyCarolina CuraJuan Carlos Ramı́rezTeresa AbateNelly M. CayoRudy ParradoZoraida Díaz BelloElsa VelázquezArturo Muñoz-CalderónNatalia Juiz2026-03-222026-03-22201310.1371/journal.pntd.0002000https://doi.org/10.1371/journal.pntd.0002000https://andeanlibrary.org/handle/123456789/43100Citaciones: 259The performing parameters of this assay encourage its application to early assessment of T. cruzi infection in cases in which serological methods are not informative, such as recent infections by oral contamination or congenital transmission or after transplantation with organs from seropositive donors, as well as for monitoring Chagas disease patients under etiological treatment.enChagas diseaseTaqManTrypanosoma cruziReal-time polymerase chain reactionMultiplexVirologyBiologyPolymerase chain reactionOutbreakDNA extractionarticle