Andrés Felipe Lizcano-SalasJesús Camilo Jacome-GarcíaDiego Riaño-JiménezMarcela Guevara‐Suarez2026-03-222026-03-22202510.1101/2025.10.13.682240https://doi.org/10.1101/2025.10.13.682240https://andeanlibrary.org/handle/123456789/84573ABSTRACT Bumble bees ( Bombus ) are considered to be essential pollinators of a wide range of flowering plants, within both agricultural and natural ecosystems. Bombus pauloensis and Bombus pullatus are two closely related Neotropical species with a wide altitudinal and latitudinal distribution that belong to the Thoracobombus genus. To the best of our knowledge, there is no genome assembly available for any species of Neotropical Bombus . Therefore, the goal of this study is to produce high-quality genomes of B. pauloensis and B. pullatus . In order to achieve this objective, we obtained long-read sequences using the Oxford Nanopore Technologies platform. We then proceeded to assemble the genomes and annotate these assemblies. As a result, we obtained assemblies of ∼240Mb represented in 72 contigs with an N50 of ∼ 9.08Mb for Bombus pullatus and ∼239Mb represented in 66 contigs with an N50 of ∼9Mb for Bombus pauloensis . The completeness evaluated by compleasm return a score >99% for both species. It is hoped that these genomes will facilitate a more profound comprehension of the biology of Neotropical bumblebees.enGenomeBiologyContigEvolutionary biologyPollinatorSequence assemblyRange (aeronautics)PhylogeneticsGenomicsComputational biologypreprint