Yanez, Rosse MaryQuitón, RocíoRojas, ErnestoVargas, TaniaEid, DanielLetesson, Jean-JacquesRodríguez, Patricia2026-03-242026-03-2420251680-5348PMID:3983525110.26633/RPSP.2025.5https://doi.org/10.26633/RPSP.2025.5https://andeanlibrary.org/handle/123456789/100882Vol. 49, pp. e5OBJECTIVE: To validate the applicability of the Rose Bengal (RB) test, indirect IgG or IgM enzyme-linked immunosorbent assay (ELISA), and the Brucellacapt test® to the local context, determine the seroprevalence of brucellosis, and evaluate knowledge of the disease in people at risk in a dairy-producing area of La Maica, Cochabamba (Plurinational State of Bolivia). METHOD: The tests were initially validated with a group of 76 people; the final study population was 330 people, who also participated in a socio-epidemiological survey. RESULTS: Of 330 samples tested, 12.7% were positive, using either the RB or Brucellacapt test®. The indirect IgG ELISA gave false positive results, indicating that the cut-off point should be adapted to the local epidemiological situation. Almost all RB-positive cases were asymptomatic. The socio-epidemiological survey highlights a lack of information and awareness about brucellosis, with regard to its zoonotic nature, mode of transmission, and symptoms. CONCLUSION: This study revealed a high seroprevalence of brucellosis in the La Maica dairy-producing area in Cochabamba (Plurinational State of Bolivia), with many asymptomatic cases and a high number of false positives from IgG indirect ELISA. It is recommended to conduct awareness-raising campaigns on the risks of this disease and to discourage the consumption of raw milk.spaBoliviaBrucellosishealth behaviorrisk factorsseroprevalenceArtículo Científico Publicado