Multiplex Real-Time PCR Assay Using TaqMan Probes for the Identification of Trypanosoma cruzi DTUs in Biological and Clinical Samples

Carolina CuraTomás DuffyRaúl Horacio LuceroMargarita BisioJulie PéneauMatilde Jiménez‐CoelloEva MuñozMaría-José GiménezEdward Valencia AyalaSonia A. Kjos2026-03-222026-03-22201510.1371/journal.pntd.0003765https://doi.org/10.1371/journal.pntd.0003765https://andeanlibrary.org/handle/123456789/44207Citaciones: 93Typing is resolved after a single or a second round of Real-Time PCR, depending on the DTU. This format reduces carryover contamination and is amenable to quantification, automation and kit production.enTaqManTypingGenotypingChagas diseaseTrypanosoma cruziBiologyVirologyMultiplexPolymerase chain reactionMultiplex polymerase chain reactionMultiplex Real-Time PCR Assay Using TaqMan Probes for the Identification of Trypanosoma cruzi DTUs in Biological and Clinical Samplesarticle