María Lenny Rodríguez-EscobarRogéria Inês Rosa LaraMargoth Atahuachi BurgosA C.Carla MaldonadoJaume BastidaLuciana R. TalliniLaura Torras‐Claveria2026-03-222026-03-22202510.3390/life15050719https://doi.org/10.3390/life15050719https://andeanlibrary.org/handle/123456789/52045Citaciones: 2Amaryllidaceae alkaloids from the Amaryllidoideae subfamily exhibit broad pharmacological activities, including neuroprotection and anticancer effects. Galanthamine is a key compound for Alzheimer's therapy. The <i>Hippeastrum</i> genus, particularly in Bolivia, offers significant potential for novel drug discovery, emphasising the need for conservation and further phytochemical research. Twenty-seven samples from Bolivian <i>Hippeastrum</i> species were investigated in terms of their alkaloid profile and anticholinesterase activity. The phytochemical analysis of Bolivian <i>Hippeastrum</i> species via GC-MS identified 48 Amaryllidaceae alkaloids, displaying diverse structural groups with potential pharmacological significance. Lycorine- and Homolycorine-type alkaloids were predominant, particularly in <i>H. chionedyanthum</i> and <i>H. haywardii</i>, with high concentrations of lycorine, a promising anticancer compound. The species <i>H. evansiarum</i> and <i>H. mollevillquense</i> contained notable quantities of Galanthamine type alkaloids, relevant for Alzheimer's treatment. This study also highlights variability in acetylcholinesterase and butyrylcholinesterase inhibitory activities, with <i>H. lara-ricoi</i> and <i>H. haywardii</i> demonstrating strong inhibition. These findings suggest that <i>Hippeastrum</i> species are a valuable source of bioactive compounds, warranting further research into their therapeutic applications.enLycorineAmaryllidaceaePhytochemicalAlkaloidCholinesteraseButyrylcholinesteraseBiologyAmaryllidaceae AlkaloidsAcetylcholinesteraseGalantaminearticle