Elkin Sanabria‐ChanagaDulce María Menéses-RuizErick Francisco Puertas-SantamaríaFernando Manuel Mancha-MeléndezEugene BratoeffMarco A. Loza-MejíaJuan Rodrigo Salazar2026-03-222026-03-22202110.1080/07391102.2021.1969279https://doi.org/10.1080/07391102.2021.1969279https://andeanlibrary.org/handle/123456789/53145Citaciones: 1Pregnane derivatives have been studied mainly for their 5α-reductase activity. However, the anti-inflammatory activities of such compounds are still poorly explored. In the search for new anti-inflammatory agents, seven new pregnane derivatives <b>6a</b>-<b>g</b>, with cinnamic acid esters at C-3 were prepared and fully characterized. The anti-inflammatory activity of compounds was assessed in TPA induced mice ear model. From them, compound <b>6 b</b> was the most active to reduce edema, with an ED₅₀ of 0.017 mg/ear. Also, Molecular Docking and Molecular Dynamics studies were performed to identify a potential molecular target related to the inflammatory process. The in vivo results suggest that <b>6 b</b> could be a potent anti-inflammatory compound, while in silico studies suggest its interaction with some critical enzymes in the inflammatory response.enIn silicoAnti-inflammatoryIn vivoChemistryCinnamic acidEnzymeStereochemistryDocking (animal)ED50Pharmacologyarticle