Browsing by Autor "Aliaga, Claudia"

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Blood meal sources of wild and domestic Triatoma infestans (Hemiptera: Reduviidae) in Bolivia: connectivity between cycles of transmission of Trypanosoma cruzi.
(2016) Buitrago, Rosio; Bosseno, Marie-France; Depickère, Stéphanie; Waleckx, Etienne; Salas, Renata; Aliaga, Claudia; Barnabé, Christian; Brenière, Simone Frédérique
BACKGROUND: Chagas disease is a major public health problem in Latin America. Its etiologic agent, Trypanosoma cruzi, is mainly transmitted through the contaminated faeces of blood-sucking insects called triatomines. Triatoma infestans is the main vector in various countries in South America and recently, several foci of wild populations of this species have been described in Bolivia and other countries. These wild populations are suspected of affecting the success of insecticide control campaigns being carried out in South America. To assess the risk that these T. infestans populations pose to human health, it is helpful to determine blood meal sources. METHODS: In the present work, blood meals were identified in various Bolivian wild T. infestans populations and in three specific areas, in both wild and intra-peridomestic populations to assess the links between wild and domestic cycles of T. cruzi transmission. PCR-HDA and sequencing of Cytb gene were used to identify these blood meal sources. RESULTS AND DISCUSSION: Fourteen vertebrate species were identified as wild blood meal sources. Of those, the most prevalent species were two Andean endemic rodents, Octodontomys gliroides (36%) and Galea musteloides (30%), while humans were the third most prevalent source (18.7%). Of 163 blood meals from peridomestic areas, more than half were chickens, and the others were generally domestic animals or humans. Interestingly, blood from wild animals was identified in triatomines captured in the peridomestic and domestic environment, and blood from domestic animals was found in triatomines captured in the wild, revealing links between wild and domestic cycles of T. cruzi transmission. CONCLUSION: The current study suggests that wild T. infestans attack humans in the wild, but is also able to bite humans in domestic settings before going back to its natural environment. These results support the risk to human health posed by wild populations of T. infestans.
Putative panmixia in restricted populations of Trypanosoma cruzi isolated from wild Triatoma infestans in Bolivia.
(2013) Barnabe, Christian; Buitrago, Rosio; Bremond, Philippe; Aliaga, Claudia; Salas, Renata; Vidaurre, Pablo; Herrera, Claudia; Cerqueira, Frédérique; Bosseno, Marie-France; Waleckx, Etienne; Breniere, Simone Frédérique
Trypanosoma cruzi, the causative agent of Chagas disease, is subdivided into six discrete typing units (DTUs; TcI-TcVI) of which TcI is ubiquitous and genetically highly variable. While clonality is the dominant mode of propagation, recombinant events play a significant evolutive role. Recently, foci of wild Triatoma infestans have been described in Bolivia, mainly infected by TcI. Hence, for the first time, we evaluated the level of genetic exchange within TcI natural potentially panmictic populations (single DTU, host, area and sampling time). Seventy-nine TcI stocks from wild T. infestans, belonging to six populations were characterized at eight microsatellite loci. For each population, Hardy-Weinberg equilibrium (HWE), linkage disequilibrium (LD), and presence of repeated multilocus genotypes (MLG) were analyzed by using a total of seven statistics, to test the null hypothesis of panmixia (H0). For three populations, none of the seven statistics allowed to rejecting H0; for another one the low size did not allow us to conclude, and for the two others the tests have given contradictory results. Interestingly, apparent panmixia was only observed in very restricted areas, and was not observed when grouping populations distant of only two kilometers or more. Nevertheless it is worth stressing that for the statistic tests of "HWE", in order to minimize the type I error (i. e. incorrect rejection of a true H0), we used the Bonferroni correction (BC) known to considerably increase the type II error ( i. e. failure to reject a false H0). For the other tests (LD and MLG), we did not use BC and the risk of type II error in these cases was acceptable. Thus, these results should be considered as a good indicator of the existence of panmixia in wild environment but this must be confirmed on larger samples to reduce the risk of type II error.
Supplementary Data and Materials for: Statistical Analysis of Larval Postspiracular Filament Length in Anopheles pseudopunctipennis
(European Organization for Nuclear Research, 2025) Lardeux, Frédéric; Vasquez, Deysi; Lardeux, Rosenka; Aliaga, Claudia; Garcia, Lineth; Torrez, Libia
This repository provides the complete dataset and all scripts used in the study “Statistical analysis of larval postspiracular filament length reveals continuous variation in Bolivian Anopheles pseudopunctipennis (Diptera: Culicidae)”, recommended by PCI Zoology.It includes the raw morphometric measurements, transformed datasets used for statistical modelling, all R scripts used for data processing and analyses, and the full set of outputs generated during the workflow. These materials enable full reproducibility of the study and allow further investigation of caudal filament variation across populations from Mataral and El Chaco (Bolivia). Contents Raw data: original morphometric measurements of larval postspiracular filaments and head-collar length. Processed datasets: outputs of measurement verification, allometric correction, and data preparation for downstream analyses. R scripts (8 scripts): measurement validation, allometry correction, site comparison, descriptive statistics, distribution fitting, mixture modelling, outlier detection, and tail-analysis procedures. Outputs: tables and figures associated with each analysis step for both sites. Supplementary tables: reassigned measurements and Pickands moment estimates for Mataral and El Chaco. Repository structure data/ (raw and transformed datasets) scripts/ (colas_1_mean.R to colas_8_tail_analysis.R) outputs/ (results for Mataral and El Chaco, structured by script) Supplementary_Tables/ (CSV files referenced in the manuscript) README.txt (detailed documentation) Data descriptionDatasets include individual larval identifiers, site information, left and right filament lengths, head-collar length, and all allometry-corrected variables (ratios, log-ratios, OLS/RLM/GAM residuals, SMA-derived predictions). Variable names are consistent across files. Software and reproducibilityAnalyses were performed in R (≥ 4.3.0). All required packages are listed in the repository documentation. Scripts are fully executable in the order described, and each step generates structured outputs. LicenseAll files are distributed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. CitationIf using these data or scripts, please cite:Lardeux F., Vasquez D., Lardeux R., Aliaga C., Garcia L., Torrez L. (2025). Statistical analysis of larval postspiracular filament length reveals continuous variation in Bolivian Anopheles pseudopunctipennis (Diptera: Culicidae). PCI Zoology.(See repository for DOI.)
Supplementary Data and Materials for: Statistical Analysis of Larval Postspiracular Filament Length in Anopheles pseudopunctipennis
(European Organization for Nuclear Research, 2025) Lardeux, Frédéric; Vasquez, Deysi; Lardeux, Rosenka; Aliaga, Claudia; Garcia, Lineth; Torrez, Libia
This repository provides the complete dataset and all scripts used in the study “Statistical analysis of larval postspiracular filament length reveals continuous variation in Bolivian Anopheles pseudopunctipennis (Diptera: Culicidae)”, recommended by PCI Zoology.It includes the raw morphometric measurements, transformed datasets used for statistical modelling, all R scripts used for data processing and analyses, and the full set of outputs generated during the workflow. These materials enable full reproducibility of the study and allow further investigation of caudal filament variation across populations from Mataral and El Chaco (Bolivia). Contents Raw data: original morphometric measurements of larval postspiracular filaments and head-collar length. Processed datasets: outputs of measurement verification, allometric correction, and data preparation for downstream analyses. R scripts (8 scripts): measurement validation, allometry correction, site comparison, descriptive statistics, distribution fitting, mixture modelling, outlier detection, and tail-analysis procedures. Outputs: tables and figures associated with each analysis step for both sites. Supplementary tables: reassigned measurements and Pickands moment estimates for Mataral and El Chaco. Repository structure data/ (raw and transformed datasets) scripts/ (colas_1_mean.R to colas_8_tail_analysis.R) outputs/ (results for Mataral and El Chaco, structured by script) Supplementary_Tables/ (CSV files referenced in the manuscript) README.txt (detailed documentation) Data descriptionDatasets include individual larval identifiers, site information, left and right filament lengths, head-collar length, and all allometry-corrected variables (ratios, log-ratios, OLS/RLM/GAM residuals, SMA-derived predictions). Variable names are consistent across files. Software and reproducibilityAnalyses were performed in R (≥ 4.3.0). All required packages are listed in the repository documentation. Scripts are fully executable in the order described, and each step generates structured outputs. LicenseAll files are distributed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. CitationIf using these data or scripts, please cite:Lardeux F., Vasquez D., Lardeux R., Aliaga C., Garcia L., Torrez L. (2025). Statistical analysis of larval postspiracular filament length reveals continuous variation in Bolivian Anopheles pseudopunctipennis (Diptera: Culicidae). PCI Zoology.(See repository for DOI.)