Supplementary Data and Materials for: Statistical Analysis of Larval Postspiracular Filament Length in Anopheles pseudopunctipennis

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European Organization for Nuclear Research

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This repository provides the complete dataset and all scripts used in the study “Statistical analysis of larval postspiracular filament length reveals continuous variation in Bolivian Anopheles pseudopunctipennis (Diptera: Culicidae)”, recommended by PCI Zoology.It includes the raw morphometric measurements, transformed datasets used for statistical modelling, all R scripts used for data processing and analyses, and the full set of outputs generated during the workflow. These materials enable full reproducibility of the study and allow further investigation of caudal filament variation across populations from Mataral and El Chaco (Bolivia). Contents Raw data: original morphometric measurements of larval postspiracular filaments and head-collar length. Processed datasets: outputs of measurement verification, allometric correction, and data preparation for downstream analyses. R scripts (8 scripts): measurement validation, allometry correction, site comparison, descriptive statistics, distribution fitting, mixture modelling, outlier detection, and tail-analysis procedures. Outputs: tables and figures associated with each analysis step for both sites. Supplementary tables: reassigned measurements and Pickands moment estimates for Mataral and El Chaco. Repository structure data/ (raw and transformed datasets) scripts/ (colas_1_mean.R to colas_8_tail_analysis.R) outputs/ (results for Mataral and El Chaco, structured by script) Supplementary_Tables/ (CSV files referenced in the manuscript) README.txt (detailed documentation) Data descriptionDatasets include individual larval identifiers, site information, left and right filament lengths, head-collar length, and all allometry-corrected variables (ratios, log-ratios, OLS/RLM/GAM residuals, SMA-derived predictions). Variable names are consistent across files. Software and reproducibilityAnalyses were performed in R (≥ 4.3.0). All required packages are listed in the repository documentation. Scripts are fully executable in the order described, and each step generates structured outputs. LicenseAll files are distributed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. CitationIf using these data or scripts, please cite:Lardeux F., Vasquez D., Lardeux R., Aliaga C., Garcia L., Torrez L. (2025). Statistical analysis of larval postspiracular filament length reveals continuous variation in Bolivian Anopheles pseudopunctipennis (Diptera: Culicidae). PCI Zoology.(See repository for DOI.)

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