Browsing by Autor "Ana Gloria Gil"

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Actividad biológica in vitro del gel de quitosano sobre candida albicans
(2013) Ana Gloria Gil; Anabella Núñez; Gladys Josefina Velazco Viloria; Clara Díaz; Judith Velasco; Anajulia González Betancourt
The increase in infections caused by Candida, limitations in treatments, in addition to drug resistance, has prompted the search for new natural therapeutic approaches, such as chitosan, a biopolymer derived from chitin, which has proven antibacterial, antifungal and anti-inflammatory. Since yeast is one of the most common oral diseases of the oral cavity, the objective of this study is to evaluate the in vitro biological activity of chitosan gel on Candida albicans, determining the minimum inhibitory concentration (MIC) of the gel and setting the change of microscopic morphology of Candida albicans. For this study, descriptive and experimental design, we used a reference strain CDC-B385 Candida albicans, which was cultured for 24-48 hours malt extract broth and 2% chitosan at different concentrations (0.25, 0, 50, 0.75, 1, 1.25, 1.50 mg / ml) also established a growth control, positive control and negative control. Subsequently 100ul of each tube cultured in Petri dishes and incubated at 37° C for 24 hours, to count the colony forming units. Tubes were then taken with and without addition of chitosan, which were centrifuged and observed under the light microscope. It was observed that chitosan inhibited the fungal growth to an MIC of 1mg/ml, showing deformation of the fungal cell and absence of pseudohyphae, proving that this biomaterial is highly effective against Candida albicans.
Minimizing creatine kinase variability in rats for neuromuscular research purposes
(SAGE Publishing, 2008) María Goicoechea; Felipe Cia; Claudio José; A. Asensio; José Ignacio Emparanza; Ana Gloria Gil; Adela López de Ceráin; P Aldazábal; Margarita Azpitarte; David Otaegui
Rat serum or plasma creatine kinase (CK) activity is widely used to evaluate myopathic processes, to test the myotoxicity of different drugs, or to analyse the benefits of emerging gene therapies in some neuromuscular disorders. However, great variability is found in this determination. The aim of this study has been to control some factors of variation in order to reduce variability and increase the reproducibility of analytical data. 8-10-week-old Wistar-Han rats were used. The study consisted of four sequential phases. Phase I aimed to analyse the effect of ether and isoflurane as anaesthetic drugs. The objective of Phase II was to evaluate bleeding rats via retro-orbital sinus vs. tail vein. Phases III and IV were designed as two separate, repeated measure experiments on two factors: habituation to laboratory handling procedures in Phase III and gender in Phase IV. The repeated factor was the storage temperature of blood sample prior to centrifugation. Ether did not significantly increased the CK value. Using isoflurane, getting rats accustomed to laboratory handling procedures and whole blood refrigeration prior to centrifugation and serum separation resulted in statistically significant reduction in CK value and variability. Male rats showed significantly higher values than female rats. In the light of our findings, CK value and variability in rats may be minimized by choosing tail vein as site of bleeding, getting rats accustomed to laboratory handling procedures and maintaining whole blood refrigerated until centrifugation and serum separation.