Actividad biológica in vitro del gel de quitosano sobre candida albicans

Abstract

The increase in infections caused by Candida, limitations in treatments, in addition to drug resistance, has prompted the search for new natural therapeutic approaches, such as chitosan, a biopolymer derived from chitin, which has proven antibacterial, antifungal and anti-inflammatory. Since yeast is one of the most common oral diseases of the oral cavity, the objective of this study is to evaluate the in vitro biological activity of chitosan gel on Candida albicans, determining the minimum inhibitory concentration (MIC) of the gel and setting the change of microscopic morphology of Candida albicans. For this study, descriptive and experimental design, we used a reference strain CDC-B385 Candida albicans, which was cultured for 24-48 hours malt extract broth and 2% chitosan at different concentrations (0.25, 0, 50, 0.75, 1, 1.25, 1.50 mg / ml) also established a growth control, positive control and negative control. Subsequently 100ul of each tube cultured in Petri dishes and incubated at 37° C for 24 hours, to count the colony forming units. Tubes were then taken with and without addition of chitosan, which were centrifuged and observed under the light microscope. It was observed that chitosan inhibited the fungal growth to an MIC of 1mg/ml, showing deformation of the fungal cell and absence of pseudohyphae, proving that this biomaterial is highly effective against Candida albicans.