XYLANASE PRODUCTION USING BARLEY STRAW BY BACILLUS SP. LB-4 ISOLATED FROM LAGUNA BLANCA, POTOSI-BOLIVIA

Abstract

Estudio sobre la producción de enzimas xilanolíticas en bacterias colectadas en Laguna Blanca, Potosi-Bolivia. La caracterización parcial de las enzimas producidas que mostraron una máxima actividad a un pH 9 a una concentración de NaCl de 50°C y 0% (w/v) exhibiendo así propiedades termo-alkalitolerantes. El análisis de la secuencia del gen 16S rDNA mostró un 99% de similaridad entre la cepa LB-4 y Bacillus pumilus (embAJ494727).

Bacterial strains isolated from water samples collected from Laguna Blanca, Potosi-Bolivia and deposited at Biotechnology Center, Cochabamba-Bolivia, were subjected to xylanolytic enzyme production studies. Among these, 20 strains were selected as positive in xylanolytic enzyme production in liquid medium, using barley straw as sole carbon source and subjected to morphological and biochemical studies. Enzyme production in liquid medium identified strain LB-4 as the best enzyme producer with 10 U/ml xylanolytic activity. Partial characterization of the produced enzyme revealed its display maximum activity values at pH 9, 50°C y 0% (w/v) NaCl concentration, thus exhibiting thermo-alkalitolerant properties. The 16S rDNA gene sequence analysis showed 99% similarity between strain LB-4 and Bacillus pumilus (embAJ494727).