Inhibición in vitro de cepas de Candida spp., mediante el aceite esencial de muña silvestre: Una planta tradicional, de uso medicinal en zonas alto andinas del Perú
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J. Selva Andina Res. Soc.
Abstract
Las plantas medicinales han sido utilizadas durante siglos por diversas culturas como fuente de tratamiento y prevención de enfermedades, estudios etnobotánicas y científicos de la muña, adquieren relevancia debido a su potencial bioactivo contra hongos de importancia en la salud pública. El estudio se realizó en Puno-Perú (longitud: 15° 50' 15´´ O, latitud: 70° 01' 18´´ S, y altitud: 4047 msnm), con el objetivo de evaluar la inhibición in vitro del aceite esencial de la muña silvestre sobre cepas de Candida spp. Se realizó un estudio experimental in vitro, se evaluaron 90 halos inhibitorios a diferentes concentraciones, mediante el método de dilución en agar. Los grupos experimentales fueron concentraciones de muña al: 25, 50, 100, 150, 200 y 250 % (Grupos experimentales) un grupo experimental fluconazol (grupo positivo) y un grupo experimental agua destilada (grupo negativo). Los grupos experimentales presentaron halos inhibitorios que fueron al 25 % (3.5±1.5 mm), 50 % (11.1±0.6 mm), 100 % (15.8±0.7 mm), 150 % (19.1±0.7 mm), 200 % (24.1±0.5 mm), 250 % (29.3±0.6 mm), fluconazol (25.5±0.6 mm) y para agua destilada, no se observó halos inhibitorios, al no tener ningún componente fitoquímico inhibidor en su composición. Se observó que la concentración al 250 % presento un halo inhibitorio superior en comparación a los grupos experimentales y el fluconazol, esto se explica por la mayor concentración de metabolitos secundarios presentes en una concentración superior.
Medicinal plants have been used for centuries by various cultures as a source of treatment and prevention for diseases. Ethnobotanical and scientific studies of "muña" have gained significance due to its bioactive potential against fungi of public health importance. The study was conducted in Puno, Peru (longitude: 15° 50' 15'' W, latitude: 70° 01' 18'' S, altitude: 4047 meters above sea level), with the aim of evaluating the in-vitro inhibition of wild mountain mint essential oil on Candida spp., strains. An experimental in-vitro study was carried out, where 90 inhibitory halos were evaluated at different concentrations using the agar dilution method. The experimental groups consisted of concentrations of mountain mint at: 25, 50, 100, 150, 200, and 250 % (experimental groups), a Fluconazole experimental group (positive group), and a distilled water experimental group (negative group). The experimental groups presented inhibitory halos at 25 % (3.5±1.5 mm), 50 % (11.1±0.6 mm), 100 % (15.8±0.7 mm), 150 % (19.1±0.7 mm), 200 % (24.1±0.5 mm), 250 % (29.3±0.6 mm), fluconazole (25.5±0.6 mm), and for distilled water, no inhibitory halos were observed, as it did not have any inhibitory phytochemical components in its composition. It was observed that the concentration at 250 % presented a superior inhibitory halo compared to the experimental groups and Fluconazole, this is explained by the higher concentration of secondary metabolites present in a higher concentration.
Medicinal plants have been used for centuries by various cultures as a source of treatment and prevention for diseases. Ethnobotanical and scientific studies of "muña" have gained significance due to its bioactive potential against fungi of public health importance. The study was conducted in Puno, Peru (longitude: 15° 50' 15'' W, latitude: 70° 01' 18'' S, altitude: 4047 meters above sea level), with the aim of evaluating the in-vitro inhibition of wild mountain mint essential oil on Candida spp., strains. An experimental in-vitro study was carried out, where 90 inhibitory halos were evaluated at different concentrations using the agar dilution method. The experimental groups consisted of concentrations of mountain mint at: 25, 50, 100, 150, 200, and 250 % (experimental groups), a Fluconazole experimental group (positive group), and a distilled water experimental group (negative group). The experimental groups presented inhibitory halos at 25 % (3.5±1.5 mm), 50 % (11.1±0.6 mm), 100 % (15.8±0.7 mm), 150 % (19.1±0.7 mm), 200 % (24.1±0.5 mm), 250 % (29.3±0.6 mm), fluconazole (25.5±0.6 mm), and for distilled water, no inhibitory halos were observed, as it did not have any inhibitory phytochemical components in its composition. It was observed that the concentration at 250 % presented a superior inhibitory halo compared to the experimental groups and Fluconazole, this is explained by the higher concentration of secondary metabolites present in a higher concentration.
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Vol. 15, No. 2