Purificación de fibroblastos gingivales a partir de tejido de la mucosa bucal

Abstract

Fibroblasts are widely used in regenerative medicine, as key elements in the restoration of the gingival tissue and facial\ntreatments. The aim of this study was to standardize in our laboratory a method to obtain highly pure fibroblasts from\ngingival tissue. In order to do that, cells were mechanically disaggregated from gingival tissue and separated by ficoll\nhypaque density gradients. This procedure was performed several times yielding an enriched culture of adherent cells,\nwhere 51% resulted positive for the fibroblast marker ER-TR7. Our results indicate that the method used, allow to obtain\nfibroblasts from gingival tissue; cells with great potential in the field of regenerative medicine and it represents, in our\ncommunity, the first step to implement therapeutic strategies that will allow the restoration of damaged tissues,\nparticularly in the oral mucosa.