Anti-angiogenic properties of "Makahiya", Mimosa pudica L. (Fabaceae) aqueous root extracts

Abstract

This study was conducted to determine the anti-angiogenic effects of different concentrations of mimosine, flavonoids, tannins, etc. from aqueous root extract (ARE) of Mimosa pudica L. (Fabaceae) on the chorioallantoic membrane (CAM) of developing duck embryo. The extraction method was done by air-drying, grounding and decoction. The AREs of <em>M. pudica</em> were prepared at different concentrations (10%, 20% and 30%). These were administered on the CAM of the 10-day old duck embryos. After 48 h upon administration of the extracts, the secondary branching points on the CAM are counted and compared with the control groups and with each of the experimental groups. The results reveal that AREs of <em>M. pudica</em> inhibited anti-angiogenesis. Results show that 10%, 20% and 30% concentrations have significantly yielded anti-angiogenic results. There is a significant difference between all the control groups and the experimental groups indicating that as the concentration increases, the branching point of the embryos decreases. This suggests the potential use of the <em>M. pudica</em> root extract for further studies on abnormal angiogenesis and evasion of apoptosis as hallmarks in cancer treatment.