Interaction of isoleucine with metal ions in chemical and biological systems

Abstract

The stability constants of Zn2+, Cd2+ and Fe2+ complexes with isoleucine were determined by paper ionophoretic technique. The present method is based on the movement of a spot of metal ions in an electric field at various pHs of background electrolyte. A graph of pH versus mobility was used to obtain information on the binary complexes and to calculate its stability constants. Using this method, the stability constants of binary complexes metal(II) -isoleucine have been determined to be (7.24 ± 0.01, 3.48 ± 0.04); (6.90 ± 0.01, 3.25 ± 0.11) and (6.50 ± 0.03, 2.97 ± 0.07) (logarithm stability constant values) for Zn(II), Cd (II) and Fe(II) complexes respectively at ionic strength 0.01 Mol L-1 and a temperature of 35°C.The stability constants of Zn2+, Cd2+ and Fe2+ complexes with isoleucine were determined by paper ionophoretic technique. The present method is based on the movement of a spot of metal ions in an electric field at various pHs of background electrolyte. A graph of pH versus mobility was used to obtain information on the binary complexes and to calculate its stability constants. Using this method, the stability constants of binary complexes metal(II) -isoleucine have been determined to be (7.24 ± 0.01, 3.48 ± 0.04); (6.90 ± 0.01, 3.25 ± 0.11) and (6.50 ± 0.03, 2.97 ± 0.07) (logarithm stability constant values) for Zn(II), Cd (II) and Fe(II) complexes respectively at ionic strength 0.01 Mol L-1 and a temperature of 35°C.