Aflatoxina b1 de aspergillus spp generado en arroz, su detección y cuantificación por métodos fluorométricos y hplc
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Rev. Bol. Quim
Abstract
Las aflatoxinas son producidas por cepas toxigénicas de Aspergillus spp como parte de sus productos metabólicos. Su toxicidad causa graves daños a la salud que los hacen ser considerados carcinógenos potentes. Existen cepas de Aspergillus que en sustratos como el arroz crecen y producen micotoxinas a 30 ° C. Se desarrollaron dos métodos analíticos para la detección y cuantificación de la aflatoxina B1; Se construyeron curvas de calibración para validar estos métodos. La producción de aflatoxina B1 se desarrolló mediante la cepa 114 QD (Aspergillus spp) en un medio que contenía 50 y 20 gramos de arroz emulando, cuando fue posible, las condiciones de almacenamiento durante el período de incubación. El análisis de 50 y 20 gramos de extractos de arroz por cromatografía líquida de alto rendimiento (HPLC) dio concentraciones promedio de aflatoxina B1 de 94, 93 y 24,64 ppb respectivamente. Por espectroscopia de fluorescencia se determinaron concentraciones promedio de 133, 37 y 27, 23 ppb. La concentración de aflatoxina Bl obtenida por ambos métodos analíticos muestra una diferencia significativa (P <0, 05) con respecto a la cantidad de sustrato, siendo mayor la concentración de aflatoxina B1 con mayor cantidad de arroz en el medio, la sensibilidad de estos métodos permite Detectar esta toxina a partir de 6 ppb.
Aflatoxins are produced by toxigenic strains of Aspergillus spp as a part of their metabolic producís. Its toxicity causes severe health damage which makes them to be considered potent carcinogens. There are Aspergillus strains that in substrates such as rice (Oryza sativa) grow and produce mycotoxins at 30 °C. Two analytical methods for the detection and quantification of aflatoxin B1 were developed; calibration curves were constructed to validate these methods. Aflatoxin Bl production was develop by strain 114 QD (Aspergillus spp.) in a médium containing 50 and 20 g of rice emulating when possible the storage conditions during incubation period. The analysis of 50 and 20 g of rice extracts by high performance liquid chromatography (HPLC) gave average concentrations of aflatoxin Bl of 94, 93 and 24,64 ppb respectively. By fluorescence spectroscopy average concentrations of 133, 37 and 27, 23 ppb were determined. Aflatoxin Bl concentrations obtained by both analytical methods show a significant difference (P <0, 05) with respect to the amount of substrate, being higher the concentration of aflatoxin B1 with greater amount of rice in the médium, the sensitivity of these methods allows to detect this toxin from 6 ppb.
Aflatoxins are produced by toxigenic strains of Aspergillus spp as a part of their metabolic producís. Its toxicity causes severe health damage which makes them to be considered potent carcinogens. There are Aspergillus strains that in substrates such as rice (Oryza sativa) grow and produce mycotoxins at 30 °C. Two analytical methods for the detection and quantification of aflatoxin B1 were developed; calibration curves were constructed to validate these methods. Aflatoxin Bl production was develop by strain 114 QD (Aspergillus spp.) in a médium containing 50 and 20 g of rice emulating when possible the storage conditions during incubation period. The analysis of 50 and 20 g of rice extracts by high performance liquid chromatography (HPLC) gave average concentrations of aflatoxin Bl of 94, 93 and 24,64 ppb respectively. By fluorescence spectroscopy average concentrations of 133, 37 and 27, 23 ppb were determined. Aflatoxin Bl concentrations obtained by both analytical methods show a significant difference (P <0, 05) with respect to the amount of substrate, being higher the concentration of aflatoxin B1 with greater amount of rice in the médium, the sensitivity of these methods allows to detect this toxin from 6 ppb.
Description
Vol. 35, No. 5