Extracto hidroalcohólico liofilizado de Passiflora edulis y Zea mays L, como potencial hipotensor arterial e hipocolesterolemia en Mus musculus hipertensos inducidos
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J.Selva Andina Anim. Sci.
Abstract
Las hojas del Zea mays L y Passiflora edulis tienen multipropósito para la medicina convencional por sus compuestos bioactivos, sin embargo, su uso en modelos animales carece de demostración y validación biomédica, de ahí el objetivo fue evaluar el efecto del extracto liofilizado de P. edulis Sims y Z. mays L como potencial hipotensor arterial e inducir la hipocolesterolemia en ratones albino suizo hipertensos. Se utilizaron 48 ratones albino suizos de 8 semanas dividiéndose en 4 grupos: G1- maracuyá (50 mg [n= 4], 100 mg [n= 4] y 200 mg [n= 4]), G2- maíz morado (50 mg [n= 4], 100 mg [n= 4] y 200 mg [n= 4]) y G3-controles: control negativo (agua destilada [n= 12]), G4-control positivo (N-nitro-L-arginina metíl éter (L-NAME) [n= 12]). Los ratones fueron sometidos en ayunas para evaluar la presión arterial sistólica (PAS), presión arterial diastólica (PAD), presión arterial media (PAM), glucosa y colesterol en estado basal y post inducción con L-NAME mediante método indirecto. A 4 semanas de estudio los animales del G1-maracuyá y G2-maíz morado mostraron efecto antihipertensivo e hipocolesterolemia significativo (p<0.05) resultando la concentración 200 mg como óptimo reductor y estabilizador de PAS, PAD, PAM, glucosa y colesterol. En conclusión, el extracto liofilizado de hojas de P. edulis (maracuyá) y Z. mays L (maíz morado) a 200 mg de concentración demostraron ser excelentes antihipertensivos e hipocolesterolemia en ratones albino suizo hipertensos.
The leaves of Zea mays L and Passiflora edulis have multipurpose for conventional medicine for their bioactive compounds, however, their use in animal models lacks demonstration and biomedical validation, hence the objective was to evaluate the effect of lyophilized extract of P. edulis Sims and Z. mays L as a potential arterial hypotensive and induce hypocholesterolemia in hypertensive Swiss albino mice. Forty-eight 8-week-old Swiss albino mice were used by dividing into 4 groups: G1- passion fruit (50 mg [n= 4], 100 mg [n= 4], and 200 mg [n= 4]), G2-purple corn (50 mg [n= 4], 100 mg [n= 4], and 200 mg [n= 4]), and G3-controls: negative control (distilled water [n= 12]), G4-positive control (N-nitro-L-arginine methyl ether (L-NAME) [n= 12]). Mice were fasted to assess systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP), glucose and cholesterol at baseline and post induction with L-NAME by indirect method. At 4 weeks of study the animals of G1-passion fruit and G2-purple corn showed significant antihypertensive and hypocholesterolemia effect (p<0.05) resulting the concentration 200 mg as optimal reducer and stabilizer of SBP, DBP, MAP, glucose and cholesterol. In conclusion, the lyophilized extract of P. edulis (passion fruit) and Z. mays L (purple corn) leaves at 200 mg concentration showed excellent antihypertensive and hypocholesterolemia effects in hypertensive Swiss albino mice.
The leaves of Zea mays L and Passiflora edulis have multipurpose for conventional medicine for their bioactive compounds, however, their use in animal models lacks demonstration and biomedical validation, hence the objective was to evaluate the effect of lyophilized extract of P. edulis Sims and Z. mays L as a potential arterial hypotensive and induce hypocholesterolemia in hypertensive Swiss albino mice. Forty-eight 8-week-old Swiss albino mice were used by dividing into 4 groups: G1- passion fruit (50 mg [n= 4], 100 mg [n= 4], and 200 mg [n= 4]), G2-purple corn (50 mg [n= 4], 100 mg [n= 4], and 200 mg [n= 4]), and G3-controls: negative control (distilled water [n= 12]), G4-positive control (N-nitro-L-arginine methyl ether (L-NAME) [n= 12]). Mice were fasted to assess systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP), glucose and cholesterol at baseline and post induction with L-NAME by indirect method. At 4 weeks of study the animals of G1-passion fruit and G2-purple corn showed significant antihypertensive and hypocholesterolemia effect (p<0.05) resulting the concentration 200 mg as optimal reducer and stabilizer of SBP, DBP, MAP, glucose and cholesterol. In conclusion, the lyophilized extract of P. edulis (passion fruit) and Z. mays L (purple corn) leaves at 200 mg concentration showed excellent antihypertensive and hypocholesterolemia effects in hypertensive Swiss albino mice.
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Vol. 10, No. 1