Actividad antifúngica de compuestos volátiles producidos por especies endófitas de Bacillus sobre Moniliophthora roreri H.C Evans et al.

Abstract

The aim of this work was to determine the antifungal activity of Bacillus subtilis 9-2, Bacillus mycoides 29-2, Bacillus sp. 33 and 72, Bacillus cereus 45-1, 51-2, 53-4 and Bacillus amyloliquefaciens 24 on mycelial growth and sporulation of the phytopathogenic fungus Moniliophthora roreri H.C Evans et al. The confrontation experiments were carried out in Petri dishes with two compartments. PDA culture medium was added to one side of the plate and TSA to the other. The bacterial strains were individually inoculated (100 µl; 1×10 8 CFU ml -1 ) on the face of the cap with TSA culture medium; while on the other lid with PDA culture medium, a mycelial disk (5 mm in diameter) of the strain of the phytopathogenic fungus was placed. Sterile distilled water was used as a control. All plates were sealed with Parafilm and incubated at 28°C for 15 days. The experiments were repeated twice. All strains of bacteria produced volatile compounds that inhibited mycelial growth and the formation of M. roreri spores. B. subtilis 9-2, B. mycoides 29-2, B. sp. 33, Bacillus cereus 45-1, 51-2 and Bacillus amyloliquefaciens 24 inhibited mycelial growth and sporulation of M. roreri by more than 60%. These findings offer new possibilities to control frosty pod rot caused by M. roreri using Bacillus endophytes strains producing antifungal volatile compounds.