Acción inhibitoria del aceite esencial de Plumbago scandens L. en promastigotes de Leishmania major like
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Vive Rev. Salud
Abstract
Introducción: La leishmaniasis es una enfermedad parasitaria causada por protozoos del género Leishmania, transmitida por la picadura de insectos flebótomos. Esta enfermedad afecta a millones de personas en todo el mundo, especialmente en áreas tropicales y subtropicales. Objetivo: Evaluar el efecto leishmanicida del aceite esencial de Plumbago scandens L en promastigotes axénicos de Leishmania majo like. Materiales y métodos: Se llevó a cabo un estudio experimental donde primordialmente se obtuvieron cultivos de promastigotes de L. major like y el aceite esencial de P. scandens L, seguido a esto se realizaron 8 diluciones seriadas a probar con concentraciones de 120, 60, 30, 15, 7.5, 3.75, 1.87 y 0.9 µg/ml contra los promastigotes, la viabilidad parasitaria se midió por medio del ensayo de MTT (bromuro de 3-(4,5- dimetiltiazol-2-ilo)-2,5-difeniltetrazol) y la confirmación de la viabilidad se realizó por microscopia. Resultados: El porcentaje de inhibición en las concentraciones Nº3 (30 µg/ml) y Nº4 (15 µg/ml) arrojaron resultados de 80, 7 y 85.1% respectivamente indicando que hay una elevada acción del aceite esencial usado contra los promatigotes de Leishmania, en cuanto a las demás concentraciones usadas. El resultado varió en un 70% a excepción de las primeras concentraciones Nº1 y 2 que por la intensidad del aceite esencial se presentaron limitaciones técnicas con el uso de espectrofotómetro. Conclusiones: el aceite esencial de P. scandens L tuvo un efecto leishmanicida alto contra los promastigotes de L. major like teniendo un efecto positivo en cuanto a la realización de más estudios especializados.
Introduction: Leishmaniasis is a parasitic disease caused by protozoa of the genus Leishmania, transmitted by the bite of sandflies. This disease affects millions of people worldwide, especially in tropical and subtropical areas. Objective: To evaluate the leishmanicidal effect of Plumbago scandens L. essential oil on axenic promastigotes of Leishmania majo like. Materials and methods: An experimental study was carried out where primarily promastigote cultures of L. major like and the essential oil of P. scandens L were obtained, followed by this 8 serial dilutions were made to test with concentrations of 120, 60, 30, 15, 7.5, 3.75, 1.87 and 0.9 µg / ml against the promastigotes, the parasite viability was measured by means of the MTT assay (3- (4,5- dimethylthiazol-2-yl) -2,5-diphenyltetrazole bromide) and the viability confirmation was made by microscopy. Results: The percentage of inhibition in concentrations Nº3 (30 µg/ml) and Nº4 (15 µg/ml) yielded results of 80, 7 and 85.1% respectively, indicating that there is a high action of the essential oil used against Leishmania promatigotes, as for the other concentrations used. The result varied by 70% except for the first concentrations Nº1 and 2, which due to the intensity of the essential oil presented technical limitations with the use of a spectrophotometer. Conclusions: the essential oil of P. scandens L had a high leishmanicidal effect against L. major like promastigotes, having a positive effect in terms of carrying out more specialized studies.
Introduction: Leishmaniasis is a parasitic disease caused by protozoa of the genus Leishmania, transmitted by the bite of sandflies. This disease affects millions of people worldwide, especially in tropical and subtropical areas. Objective: To evaluate the leishmanicidal effect of Plumbago scandens L. essential oil on axenic promastigotes of Leishmania majo like. Materials and methods: An experimental study was carried out where primarily promastigote cultures of L. major like and the essential oil of P. scandens L were obtained, followed by this 8 serial dilutions were made to test with concentrations of 120, 60, 30, 15, 7.5, 3.75, 1.87 and 0.9 µg / ml against the promastigotes, the parasite viability was measured by means of the MTT assay (3- (4,5- dimethylthiazol-2-yl) -2,5-diphenyltetrazole bromide) and the viability confirmation was made by microscopy. Results: The percentage of inhibition in concentrations Nº3 (30 µg/ml) and Nº4 (15 µg/ml) yielded results of 80, 7 and 85.1% respectively, indicating that there is a high action of the essential oil used against Leishmania promatigotes, as for the other concentrations used. The result varied by 70% except for the first concentrations Nº1 and 2, which due to the intensity of the essential oil presented technical limitations with the use of a spectrophotometer. Conclusions: the essential oil of P. scandens L had a high leishmanicidal effect against L. major like promastigotes, having a positive effect in terms of carrying out more specialized studies.
Description
Vol. 7, No. 21