Inocuidad de lactobacilos potencialmente probióticos aislados de heces de lactante y leche materna.

Abstract

Lactobacillus belongs to the Acid Lactic Bacteria group, which is known for their probiotic properties. These microorganisms are historically considered safe for their close association with food, they are inhabitants of the normal indigenous microbiota and their pathogenic potential is low. The objective of the present study was to evaluate the safety of potentially probiotic lactobacilli strains, isolated from breast-fed infant stools and breast milk in Merida, Venezuela, through a bacterial translocation assay on liver and spleen. Two strains of Lactobacillus paracasei ssp. paracasei (66 and 71) and one strain of L. rhamnosus (75) were evaluated; these strains were described as potential probiotics microorganism according previous in vitro tests. Bacteria were orally administrated to BALB/c mice for 7 days. ANOVA one way test was used to assess the statistical significance of the differences between test and control groups. There were no significant differences in bacterial growth of liver and spleen between control mice and those receiving oral administration of lactobacilli strains. Therefore these strains are likely to be safe for human consumption.