Development of Multiplex PCR Assays for Detection of Enterotoxigenic <i>Escherichia coli</i> Colonization Factors and Toxins

Abstract

ABSTRACT Four multiplex PCR assays for detection of 19 enterotoxigenic Escherichia coli (ETEC) colonization factors and an improved ETEC toxin multiplex PCR were developed and tested on Bangladeshi and Bolivian ETEC strain collections. The assays will be useful for surveillance of ETEC infections in diagnostic laboratories that have access to PCR.