Characterization and determination of antimicrobial and metal resistant profiles of Xanthomonas strains isolated from natural environments

Abstract

Three Xanthomonas strains were isolated from natural environments in Venezuela and studied morphologically, subjected to biochemical characterization and microbiologically and molecularly identified. In order to reconfirm the identity of these strains, the production of xanthan was induced for Xanthomonas campestris and Xanthomonas vesicatoria isolates, obtaining a yield of 7 and 35mg/100mL of culture, respectively; the characterization by FTIR spectroscopy of the obtained products confirmed the formation of xanthan during the growth of the strains. Characterized strains were submitted to resistance studies to 14 commercial antibiotics of clinical use as well as to 10 metal salts were carried out in Luria Bertani (LB) medium, which disturbingly confirmed these Xantho-monas strains present important percentages of resistance to the antibiotics (79%) and to the salts (50%) tested.