Microscopic evaluation of semen characteristics llama (Lama glama) cryopreserved two dilutors

Abstract

The present study was conducted at the Experimental Station Kallutaca, under the race Veterinary Medicine and Animal Husbandry of the UPEA, located in the province of Los Andes Department of La Paz at 3900 meters, whose objective was to evaluate the microscopic features semen llama (Lama glama) cryopreserved two diluters. 15 ejaculates were processed using llamas electroejaculation 12 players 4 years old on average. The test was part of a design of random blocks (DBA). The treatments were: T1. Andromed and T2. Tris fructose citric-acid-yolk-glycerol. The variables evaluated were: sperm motility (%), sperm vitality (%) and morphological abnormalities (%). By comparing the sperm variables in the post-thawed (cryopreserved) significant differences (P<0.05) between dilutors for sperm motility (%), sperm vitality (%) and morphological abnormalities (%), average values ​​obtained are found and standard deviation: T1. 0%; T2. 17.03±1.83%, T1. 3.80±0.40%, T2. 20.13±2.06% and T1. 8.40±0.64% and T2. 10.17±0.22% respectively for the dilutor Andromed Tris-citric acid and fructose-yolk-glycerol. The results of cryopreserved semen diluters both proved to be lower than those obtained in fresh semen, because the semen quality decreases as time passes cryopreservation.