Sources of Blood Meals of Sylvatic Triatoma guasayana near Zurima, Bolivia, Assayed with qPCR and 12S Cloning

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dc.contributor.authorDavid E. Lucero
dc.contributor.authorWilma Ribera
dc.contributor.authorJ.C. Pizarro
dc.contributor.authorPlaza Carlos
dc.contributor.authorLevi W. Gordon
dc.contributor.authorReynaldo Peña
dc.contributor.authorL. A. Morrissey
dc.contributor.authorDonna M. Rizzo
dc.contributor.authorLori Stevens
dc.coverage.spatialBolivia
dc.date.accessioned2026-03-22T14:13:58Z
dc.date.available2026-03-22T14:13:58Z
dc.date.issued2014
dc.descriptionCitaciones: 26
dc.description.abstractWe show that cloning of 12S PCR products, which avoids bias associated with developing primers based on a priori knowledge, detected blood meal sources not previously considered and that species-specific qPCR is more sensitive. All samples identified as positive for a specific blood meal source by the cloning assay were also positive by qPCR. However, not all samples positive by qPCR were positive by cloning. We show the power of combining the cloning assay with the highly sensitive hydrolysis probe-based qPCR assay provides a more complete picture of blood meal sources for insect disease vectors.
dc.identifier.doi10.1371/journal.pntd.0003365
dc.identifier.urihttps://doi.org/10.1371/journal.pntd.0003365
dc.identifier.urihttps://andeanlibrary.org/handle/123456789/45311
dc.language.isoen
dc.publisherPublic Library of Science
dc.relation.ispartofPLoS neglected tropical diseases
dc.sourceUniversity of Vermont
dc.subjectBlood meal
dc.subjectCloning (programming)
dc.subjectBiology
dc.subjectMolecular biology
dc.subjectSaliva
dc.titleSources of Blood Meals of Sylvatic Triatoma guasayana near Zurima, Bolivia, Assayed with qPCR and 12S Cloning
dc.typearticle

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