Aislados Proteínicos de granos altoandinos Chenopodiaceas; quinua "Chenopodium Quinoa" - Cañahua "Chenopodium Pallidicaule" por Precipitación Isoeléctrica

Abstract

The quinoa (Chenopodium Quinoa Willd) and the canihua (Chenopodium Pallidicaule Aellen), pseudocereals of the region of the Andes of South America, report an exceptional composition of amino acids. Considering these antecedents this study has the purpose of to isolate and to optimize the extraction effects and precipitation of the proteins in the point isoeléctrico in two quinoa varieties and canihua, respectively. The isolated proteic got ready by means of the extraction to pH 8,0 - 8,9 to protect the material proteic of the oxidation, an antioxidant was added in the extract watery sulphite of sodium 0,1% p/p and precipitation of the proteins to pH 4,5 at 5,3. The content of proteins is of 85,86; 83,60; 87,74; 82,68% for QIK, QST, CAK, CVT respectively with a humidity of 7,71; 8,11; 7,59; 8,10 for CVT, CAK, QIK and QST respectively, value relatively low that confer stability in the time. The solubility of the isolated proteic increases until the 45 ºC and diminish above the 45 ºC observed the denaturalization of the protein. The solubility of the proteins to pH 4,5 are smaller, being observed a higher solubility to pH different from the point isoelectric, what shows us that the method applied in the obtaining and optimization of the isolated quinoa proteic and canihua is adapted

The quinoa (Chenopodium Quinoa Willd) and the canihua (Chenopodium Pallidicaule Aellen), pseudocereals of the region of the Andes of South America, report an exceptional composition of amino acids. Considering these antecedents this study has the purpose of to isolate and to optimize the extraction effects and precipitation of the proteins in the point isoeléctrico in two quinoa varieties and canihua, respectively. The isolated proteic got ready by means of the extraction to pH 8,0 - 8,9 to protect the material proteic of the oxidation, an antioxidant was added in the extract watery sulphite of sodium 0,1% p/p and precipitation of the proteins to pH 4,5 at 5,3. The content of proteins is of 85,86; 83,60; 87,74; 82,68% for QIK, QST, CAK, CVT respectively with a humidity of 7,71; 8,11; 7,59; 8,10 for CVT, CAK, QIK and QST respectively, value relatively low that confer stability in the time. The solubility of the isolated proteic increases until the 45 ºC and diminish above the 45 ºC observed the denaturalization of the protein. The solubility of the proteins to pH 4,5 are smaller, being observed a higher solubility to pH different from the point isoelectric, what shows us that the method applied in the obtaining and optimization of the isolated quinoa proteic and canihua is adapted