In vitro response differentiation of Phaseolus vulgaris cv. ‘ICA Pijao’ and cv. ‘Tío Canela- 75’ to high temperatures

Abstract

To develop an in vitro selection protocol, it is necessary to differentiate between tolerant and susceptible cultivars to the studied factor. The present work was carried out with the objective of selecting the temperature that allows to in vitro differentiating the response of the Phaseolus vulgaris cultivars 'ICA Pijao' (susceptible) and 'Tío Canela-75' (tolerant) to high temperatures. Different temperatures (30, 35, 40, 45 and 50 °C) were applied to seeds of the two cultivars. In vitro seed germination percentage, radicle and plumule length, number of explants that formed callus and number of shoots per callus were evaluated. It was possible to differentiate between cv. ‘ICA Pijao’ (susceptible) and ‘Tío Canela- 75’ (tolerant) with the variables radicle length, plumule length, percentage of calli formed and number of regenerated shoots. It was selected 35 °C as the selective agent.