Selección de cepas de Trichoderma spp. generadoras de metabolitos secundarios de interés para su uso como promotor de crecimiento en plantas cultivadas

Abstract

The Trichoderma Trichoderma interaction of 35 strains was studied becau\nse of the fungal potential to synthesize\nSecondary Metabolites (MS), such as the 3-indoleacetic acid (IAA), by using dual confrontations in solid crop of\npotato-dextrose-agar (PDA) as a selecting method for forming strains of "defense\nbarrier" between 0.5-0.7cm with an\noptimum width selection. 20\ndual confrontations were identified. These\nwere replicated in liquid culture potato-\ndextrose broth (PDB) under constant stirring. After 9 days\nof growth, the filtration was performed to separate the\nmetabolites synthesized by the fungus mycelium formed. Th\nis substance of interest, containing the MS, was\ninoculated into two crops:\nLactuca sativa\nand Raphanus sativus. \nIt was found that the best treatments for testing are the\nLactuca sativa 3(BP-T0001-BPT0029), 4(BP-T0006-BPT0028),7(BP-\nT0007-BPT0024) confrontations and the\nindividual crop I (BP- T0028) as the best inducer strain\ns of growth and involving weight, length , root volume and\nleaf weight . For testing of\nRaphanus sativus, the best treatments are the confrontation 10 (BP- T0015 - BPT0025)\nand the individual K crop (BP-T0031) as inducers of root\nlength, diameter and bulb\nweight. The individual G crop\n(BP-T0024) and the chemical fertilizer\nQUI, inducers of leaf weight and the confrontation 3(BP- T0001-BPT0029) as inducer of root weight. Therefore, the MS synthesized by\nTrichodermaspp. MS during dual confrontations act as Growth Promoters in Plants (PGP)