Browsing by Autor "Judith Velasco"

Now showing 1 - 7 of 7

Actividad antibacteriana e hipoglicemiante de los extractos crudos del Phyllanthus Salviaefolius H.B.K. (Euphorbiaceae) de Los Andes venezolanos
(2016) Silvana Villarreal; Irama Judith Ramírez González; Mariana González-Solórzano; Judith Velasco; Tulia Mariela Díaz de Rivas; Carlos Ciangherotti; Juan Carmona Arzola
In this work the antibacterial and hypoglycemic activity of ichloromethane and methanolic extracts from the leaves of Phyllanthus salviaefolius H.B.K. (Euphorbiaceae) was studied. The antibacterial activity was determined by the diffusion method in agar against Gram positive and Gram negative bacteria. The methanolic extract presented activity against Staphylococcus aureus ATCC 25923 and Enterococcus faecalis ATCC 29212. On the other hand the dichloromethane extract presented good hypoglycemic activity at 100 mg/kg and 300 mg/kg doses. In agreement to the consulted literature it is the first time that brings the biological activity for this vegetable species.
Actividad antimicrobiana de los extractos polares de tres especies de solanum: s. hypomalocophyllum bitter, s. mamosum l y s.sycophanta dunal et dc de Venezuela
(2019) Libia del Valle Alarcón Pineda; Alexis Eduardo Peña Rangel; Alfredo Usubillaga; Judith Velasco
In this study antibacterial activity of three species of Solanum, collected in the Venezuelan Andes is reported. Antibacterial activity was measured by the diffusion in agar method using bacteria and yeasts of international reference for comparison. Hydroalcoholic extracts of roots, stems and leaves of S. mamosum L. showed respectively a CIM of 420, 260 and 120 mg/ml of activity against Staphylococcus aureous (ATCC 25923). If S. aureus was resistant to meticilina SARM 525 only stems and and leaves were active and showed CIM of 180 and 90 mg/ml respectively. A crude butanolic extract of the green berries (MDA) of S. hypomalacophyllum Bitter showed activity against SARM 525, with CIM of 30 mg/ml. On the other hand desacetoxy-solafi lidine (3) was active with a CIM of 20 mg/ml. Regarding antifungic activity MDA and compound 3 presented activity against Candida crusei (ATCC 6258), but MDA (CIM 25mg/ml) performed better than compound 3, which means that activity of MDA is associated to sinergism. Solanum sycophanta extracts were not active.
Actividad biológica in vitro del gel de quitosano sobre candida albicans
(2013) Ana Gloria Gil; Anabella Núñez; Gladys Josefina Velazco Viloria; Clara Díaz; Judith Velasco; Anajulia González Betancourt
The increase in infections caused by Candida, limitations in treatments, in addition to drug resistance, has prompted the search for new natural therapeutic approaches, such as chitosan, a biopolymer derived from chitin, which has proven antibacterial, antifungal and anti-inflammatory. Since yeast is one of the most common oral diseases of the oral cavity, the objective of this study is to evaluate the in vitro biological activity of chitosan gel on Candida albicans, determining the minimum inhibitory concentration (MIC) of the gel and setting the change of microscopic morphology of Candida albicans. For this study, descriptive and experimental design, we used a reference strain CDC-B385 Candida albicans, which was cultured for 24-48 hours malt extract broth and 2% chitosan at different concentrations (0.25, 0, 50, 0.75, 1, 1.25, 1.50 mg / ml) also established a growth control, positive control and negative control. Subsequently 100ul of each tube cultured in Petri dishes and incubated at 37° C for 24 hours, to count the colony forming units. Tubes were then taken with and without addition of chitosan, which were centrifuged and observed under the light microscope. It was observed that chitosan inhibited the fungal growth to an MIC of 1mg/ml, showing deformation of the fungal cell and absence of pseudohyphae, proving that this biomaterial is highly effective against Candida albicans.
Caracterización química y actividad antibacteriana del aceite esencial de Mangifera indica L. de tres regiones de Venezuela
(National University of Colombia, 2019) Rosa Aparicio; Judith Velasco; Rafael Paredes Uzcategui; Luís B. Rojas
Los aceites esenciales (AE) de las hojas de Mangifera indica L. recolectadas en los estados Mérida (M), Barinas (B) y Portuguesa (P), Venezuela, fueron obtenidos por el método de hidrodestilación, utilizando la trampa de Clevenger, obteniéndose 0,1 mL (0,0025%), 1,4 mL (0,035%) y 1,0 mL (0,025%), respectivamente. Los AE se caracterizaron por el método de cromatografía de gases acoplado a espectrometría de masas (CG/EM), se identificaron 30 compuestos en la muestra recolectada en M, 24 en B y 14 en P, siendo los mayoritarios en M: β-selineno (22,56%), α-gurjuneno (14,66%), β-cariofileno (10,40%), en B: β-cariofileno (36,32%), α-humuleno (22,71%), α-gurjuneno (21,43%) y en P: β-cariofileno (36,07%), α-gurjuneno (22,55%) y α-humuleno (21,24%). Debido al rendimiento, solo se determinó la actividad antibacteriana en los AE de B y P, por el método de difusión en agar con discos, frente a bacterias de referencia internacional (Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 23357 y Pseudomonas aureginosa ATCC 27853). Ambos AE inhibieron el desarrollo de S. aureus y E. faecalis con una concentración inhibitoria mínima de 200 µL/mL y 300 µL/mL, respectivamente. Este es el primer estudio comparativo y actividad antibacteriana del aceite esencial obtenido de las hojas de M. indica L. de tres regiones de Venezuela.
COMPOSICIÓN QUÍMICA Y ACTIVIDAD ANTIBACTERIANA DEL ACEITE ESENCIAL DE Zingiber officinale DEL MUNICIPIO INDEPENDENCIA ESTADO TÁCHIRA
(2010) Libia Alarcón; Aránzazu Peña; Judith Velasco; Alfredo Usubillaga
Elpresente trabajo comprende elestudiodela composicion quimica y actividad antibacteriana del aceite esencial de las hojas de Zingiber officinale, plantaquecreceenelestadoTachira(Venezuela).El aceite fue obtenido por hidrodestilacion usando una trampa deClevenger yfueanalizado porCGyCG- MS. Se logro identificar 21 compuestos que representan el 97,8% del total del contenido volatil. Loscompuestosencontradosmasabundantesfueron: 1,8-cineole (16, 9%), geraniol (15,1%), canfeno (12,6%),neral(11,6)yborneol(11,1%).Seevaluola actividad antibacteriana in vitro del aceite por el metodo de difusion en agar con discos, contra Staphylococcusaureus(ATCC25923), Enterococcus faecalis (ATCC 29212), Klebsiella pneumoniae (ATCC 23357), Escherichia coli (ATTC 25922) y Pseudomonas aeruginosa (ATTC 27853). El aceite deZ.officinalemostroposeeractividadcontratodos los microorganismos ensayados, con un rango de CIM entre 20 y 240 μg/ml. La actividad antibacteriana del aceite esencial deZ.officinale abajas concentraciones, esuna opcion que ofrece la medicinanaturalcontracepasbacterianas querepresentan unproblemadesaludimportante.
Composición Química y Evaluación de la Actividad Antimicrobiana del Aceite Esencial de Espeletia Schultzii Wedd (Asteraceae) Recolectada en el Estado Trujillo – Venezuela
(2016) Libia del Valle Alarcón Pineda; Alexis Peña; Judith Velasco; Alfredo Usubillaga; Billmary Z. Contreras Moreno; Luís B. Rojas; Deisy Ramírez; Rosa Aparicio
The essential oil of fresh leaves Espeletia schultzii Wedd (Asteraceae) obtained by hydrodistillation was rich in hydrocarbon monoterpenes. The analysis of its volatile components by chromatography gas-mass spectrometry (GC-MS) allowed the identification of 13 components, which constituted 100% of essential oil of which the majority were α-pinene (50.11%), β-pinene (16.28%) and β-myrcene (14.71%). The antibacterial activity of the essential oil was assessed using methods agar diffusion and broth microdilution discs against Staphylococcus aureus (ATCC 25923) Enterococcus faecalis (ATCC 29212), Salmonella typhi (CDC57), Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 23357) and Pseudomonas aeruginosa (ATCC 27853) and antifungal activity against Candida albicans (CDC-B385) and Candida krusei (ATCC 6258). This oil showed only activity antibacterial, with variation in the results according to the method used by the diffusion method agar disk inhibited by Gram positive bacteria (S. aureus and E. faecalis) with inhibition halos of 7 to 9 mm in diameter and minimum inhibitory concentration values (MIC) ranging between 280 and 580 mg / mL, using the broth microdilution method, inhibited the development of all microorganisms tested at concentrations of 10 to 100 ug / mL. These results revealed that the sensitivity of the method plays an important role in the evaluation of this oil as an antibacterial, It is more sensitive the broth microdilution method. This is the first report of the activity antibacterial essential oil obtained from the leaves of this species.
Evaluación de los parámetros seminales en hombres varicocelectomizados tratados con Lepidium meyenni (Maca)
(2019) Ricardo Lozano‐Hernández; Judith Velasco; Arlett Juárez; José E. Machado
Varicocele is the most frequent cause of male infertility which is characterized by poor seminal quality with high levels of reactive oxidative species. Surgical correction is considered the treatment of choice in this disorder and few studies focus on improving semen quality after varicocelectomy. In this study we determine the effect of Lepidium meyenii in patients with varicocele and post varicocelectomy. Semen samples were evaluated in thirty patients with infertility and varicocele grades II and III before surgery, at three and six months after surgery. At three months after surgery men were evaluated again and treated with 1,200 mg/day of L. meyenii. After three months post-surgery, an increase in sperm parameters was observed with a reduction in seminal viscosity; but at six months the improvement of the seminal parameters was more evident in treated men. Samples of patients with severe oligozoospermia improved after treatment with L. meyenii. Probably the antioxidant effect of polyphenols of maca are reducing factors of oxidative stress in the varicose testis. It is concluded that the administration of L. meyenni in patients with varicocele helps the restoration of sperm quality and probably in fertility.