Análogo de tejido conectivo a partir de fibroblastos gingivales sembrados en membranas de colágeno comercial

Abstract

Modern dentistry using guided tissue regeneration and tissue engineering in periodontal defects and gingival recession treatment, applying surgical techniques such autologous grafts have shown excellent results. The objetive was to determine the efficiency of gingival connective tissue analogs, obtained from cultured fibroblasts seeded on a commercial collagen scaffold, for grafting in Wistar rats induced gingival recession. The methodology Connective tissue analog was developed sowing a human gingival fibroblasts culture on a collagen scaffold Membracel®-O, at the same time it was induced a 2 mm gingival recession in the central incisors of three Wistar rats, lifting a Newman flap to create a marginal bone dehiscence; 45 days after, the connective analog graft was inserted in rat 2, rat 3 received a Membracel ®-O graft without cells and rat 1 didn’t received any graft leaving it as a control, finally the animals were clinically evaluated every week before euthanasia. The results: Rat 1 maintained the 2mm gingival recession, rat 2 achieved an insertion gain of 2 mm also presenting increased biotype and rat 3 presented a 1.5 mm gingival recession. It can be suggested that using Membracel ®-O to produce connective tissue analogues may be an alternative to gingival recession treatment since insertion gain was achieved with no evidence of clinical signs of rejection.