Interacciones proteína - proteína entre enzimas del glicosoma de Leishmania Mexicana Mexicana. Caracterización preliminar

Abstract

Leishmania mexicana mexicana is one of the most important etiologic agents of cutaneous leishmaniasis in the new world, which affects millions of people mostly in poor or developing countries, creating a public health problem. This parasite shares biological characteristics with Trypanosoma cruzi, Trypanosoma brucei gambiense, Trypanosoma brucei rhodesiense, among the most prominent are: the presence of glycosomes (peroxisome - like microbody), kinetoplast and RNA editing. Through gel filtration chromatography (CL6B Sepharose), we were able to characterize protein - protein interactions between enzymes of L. mexicana mexicana glycosome, obtaining preliminary interaction of subunits of the enzymes hexokinase (HK), phosphoglucose isomerase (PGI), phosphoglycerate kinase (PGK) and glyceraldehyde - 3 - phosphate dehydrogenase (GAPDH), with different oligomerization states to mammals and other Kinetoplastidae, plus the possible interaction of HK with two other yet unidentified protein. These data will be confirmed by techniques such as co - immunoprecipitation, cross - linking or two - hybrid assay, with the aim to determine the significance of these associations and their possible role in regulating the glycolytic pathway in L. Mexicana mexicana.