EVALUACIÓN DE LA TOXICIDAD AGUDA A DOSIS FIJAS DEL EXTRACTO DE WERNERIA DACTYLOPHYLLA (PUPUSA)
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Rev. Bol. Quim
Abstract
Se realizó un estudio con el objeto de evaluar el posible efecto tóxico de un extracto acuoso liofilizado de Werneria dactylophylla (Pupusa) reconocida por sus importantes propiedades farmacológicas. Para el desarrollo del mismo se utilizó el procedimiento de dosis fijas, utilizando dosis límite de 2000 mg/Kg y 5000 mg/Kg de peso corporal, administradas por vía orogástrica, realizándose observaciones durante 14 días. Los animales seleccionados fueron ratones Swiss albinos hembras con un peso comprendido entre 18 y 20 g. Los resultados demostraron la inocuidad de la planta al no observarse signos ni síntomas de toxicidad. El peso corporal se comportó acorde a la curva de crecimiento de la especie y no se apreciaron alteraciones macroscópicas en los órganos estudiados, todo lo que permite afirmar que la DL50 se ubica por encima de 5000 mg/Kg, considerándose el producto como no tóxico según el criterio de Williams. La metodología y el diseño experimental se llevó a cabo siguiendo la orientación descrita por la norma EPA (Agencia de Protección Ambiental) 870.1100, OECD 425 (Organización Económica para el Comercio y Desarrollo), que abarca las 24 naciones más desarrolladas del mundo, para la aplicación del método de las Clases de Toxicidad Aguda (CTA)
A study was conducted to evaluate the potential toxic effect of a lyophilized aqueous extract of Werneria dactylophylla (Pupusa) recognized for their important pharmacological properties. For its development we used the fixed dose procedure, using a limit dose of 2000 mg / kg and 5000 mg / kg of body weight, administered by orogastric route for 14 days performing observations. The selected animals were female albino Swiss mice weighing between 18 and 20 g. Results demonstrated the safety of the plant with no signs or symptoms of toxicity observed. Body weight behaved according to the growth curve of the species and there were no gross abnormalities in the studied organs, allowing to say that the LD50 is above 5000 mg / kg, considering the product as nontoxic as the Williams' criterion. The methodology and experimental design was conducted along the lines described by the standard EPA (Environmental Protection Agency) 870.1100, OECD 425 (Organization for Economic Trade and Development), which covers the 24 most developed nations of the world, for applying the method of Acute Toxicity Class (CTA)
A study was conducted to evaluate the potential toxic effect of a lyophilized aqueous extract of Werneria dactylophylla (Pupusa) recognized for their important pharmacological properties. For its development we used the fixed dose procedure, using a limit dose of 2000 mg / kg and 5000 mg / kg of body weight, administered by orogastric route for 14 days performing observations. The selected animals were female albino Swiss mice weighing between 18 and 20 g. Results demonstrated the safety of the plant with no signs or symptoms of toxicity observed. Body weight behaved according to the growth curve of the species and there were no gross abnormalities in the studied organs, allowing to say that the LD50 is above 5000 mg / kg, considering the product as nontoxic as the Williams' criterion. The methodology and experimental design was conducted along the lines described by the standard EPA (Environmental Protection Agency) 870.1100, OECD 425 (Organization for Economic Trade and Development), which covers the 24 most developed nations of the world, for applying the method of Acute Toxicity Class (CTA)
Description
Vol. 28, No. 2