Uso de marcadores moleculares microsatelite para el análisis de la diversidad genética de papa nativa de Bolivia
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J. Selva Andina Res. Soc.
Abstract
Bolivia posee una enorme diversidad de papa cultivada y sus parientes silvestres por ser parte del centro de origen de este cultivo; sin embargo, esa diversidad genética en la colección boliviana de papa no es bien conocida, lo que evita que sea utilizada de manera más eficiente. Con el objetivo de conocer mejor la diversidad de la colección, se realizó un estudio utilizando un set de 22 marcadores microsatélites para la caracterización molecular de seis especies de papa cultivada: Solanum tuberosum Subsp. andigena (991 accesiones), S. x ajanhuiri (56 accesiones), S. x curtilobum (78 accesiones), S. x juzepczukii (116 accesiones), S. stenotomum (228 accesiones) y S. goniocalyx (5 accesiones). Los resultados muestran que la diversidad genética varía en función de la especie, presentando en orden de mayor a menor diversidad a Solanum tuberosum Subsp. andigena, S. stenotomum, S. x curtilobum, S. x juzepczukii, S. x ajanhuiri y S. goniocalyx. En base a los datos moleculares, se encontró además alrededor de 60 accesiones potencialmente duplicadas, y se identificaron los microsatélites STM 5114, STG0010, STM 0037 y STM1104 como los más polimórficos, con valores de PIC de 0.74, 0.73, 0.72 y 0.69 respectivamente.
Bolivia has a huge diversity of cultivated potato and its wild relatives for being part of the center of origin of this crop; however, that genetic diversity in the Bolivian potato collection is not well known, preventing its more efficient use. With the aim to better understand the collection diversity, a study was conducted using a set of 22 simple sequence repeats (SSR) for molecular characterization of six species of cultivated potato: Solanum tuberosum Subsp. andigena (991accessions), S. x ajanhuiri (56 accessions), S. x curtilobum (78 accessions), S. x juzepczukii (116 accessions), S. stenotomum (228 accessions) and S. goniocalyx (7 accessions). Results show that genetic diversity varíes with the species, presenting from highest to lowest diversity to Solanum tuberosum ssp. andigena, S. stenotomum, S. x curtilobum, S. x juzepczukii, S. x ajanhuiri and S. goniocalyx. Based on molecular data, about 60 potentially duplícate accessions were also found and SSRs STM 5114, STG0010, STM 0037 and STM1104 were identified as the most polymorphic, with PIC valúes from 0.74, 0.73, 0.72 and 0.69, respectively.
Bolivia has a huge diversity of cultivated potato and its wild relatives for being part of the center of origin of this crop; however, that genetic diversity in the Bolivian potato collection is not well known, preventing its more efficient use. With the aim to better understand the collection diversity, a study was conducted using a set of 22 simple sequence repeats (SSR) for molecular characterization of six species of cultivated potato: Solanum tuberosum Subsp. andigena (991accessions), S. x ajanhuiri (56 accessions), S. x curtilobum (78 accessions), S. x juzepczukii (116 accessions), S. stenotomum (228 accessions) and S. goniocalyx (7 accessions). Results show that genetic diversity varíes with the species, presenting from highest to lowest diversity to Solanum tuberosum ssp. andigena, S. stenotomum, S. x curtilobum, S. x juzepczukii, S. x ajanhuiri and S. goniocalyx. Based on molecular data, about 60 potentially duplícate accessions were also found and SSRs STM 5114, STG0010, STM 0037 and STM1104 were identified as the most polymorphic, with PIC valúes from 0.74, 0.73, 0.72 and 0.69, respectively.
Description
Vol. 4, No. 1