Parámetros cinéticos de espermatozoides en semen fresco y crioconservado de alpaca (Vicugna pacos L.)
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J.Selva Andina Anim. Sci.
Abstract
La crioconservación de los epz de alpaca es importante para la mejora genética. El presente trabajo tuvo como propósito evaluar los parámetros cinéticos de spz en semen fresco y crioconservado en dilutores, mediante el equipo ASAC. Se utilizaron 3 alpacas machos, que fueron entrenados durante un mes en la colección de semen con la vagina artificial. El esperma se congeló empleando dos diluyentes (AndroMed comercial) y (AndroMed comercial, ácido cítrico, gentamicina y glicerol). El semen tuvo las siguientes características, volumen 1.5±0.7 mL, viscosidad 5.9±1.5, pH de 7.1, con tendencia a alcalinidad y el color que predominó fue el blanco translucido. Los promedios de parámetros cinéticos en semen fresco fueron VCL (25.8±4.2 µm/s), VSL (10.3±2.5 µm/s) y VAP (18.9±3.2 µm/s). Los parámetros cinéticos en semen crioconservado fuero el dilutor 2 VCL (8.4±1.4 µm/s), el dilutor 1 (7.2±1.2 µm/s), VSL el dilutor 2 (3.4±0.8 µm/s), dilutor 1 (2.9±0.7µm/s), VAP dilutor 2 (5.8±1.0 µm/s), dilutor 1 (4.8±0.8 µm/s). Se concluye que el dilutor 2 constituye una alternativa más para la crioconservación de semen de alpaca.
Cryopreservation of alpaca sperm is important for genetic improvement. The purpose of this work was to evaluate the sperm kinetic parameters in fresh semen and cryopreserved in dilutors, using the CASA team. 3 male alpacas were used, which were trained for a month in the semen collection with the artificial vagina. The sperm was frozen using two diluents (commercial AndroMed) and (commercial AndroMed, citric acid, gentamicin and glycerol). The semen had the following characteristics, volume 1.5±0.7 mL, viscosity 5.9±1.5, pH 7.1, with an alkalinity tendency and the color that predominated was the translucent white. The averages of kinetic parameters found in fresh semen were VCL (25.8±4.2 µm/s), VSL (10.3±2.5 µm/s) and VAP (18.9±3.2 µm/s). Kinetic parameters in cryopreserved semen showed that dilutor 2 VCL (8.4±1.4 µm/s), dilutor 1 (7.2±1.2 µm/s), VSL dilutor 2 (3.4±0.8 µm/s), dilutor 1 (2.9±0.7µm/s), VAP dilutor 2 (5.8±1.0 µm/s), dilutor 1 (4.8±0.8 µm/s). It is concluded that dilutor 2 is another alternative for cryopreservation of alpaca semen.
Cryopreservation of alpaca sperm is important for genetic improvement. The purpose of this work was to evaluate the sperm kinetic parameters in fresh semen and cryopreserved in dilutors, using the CASA team. 3 male alpacas were used, which were trained for a month in the semen collection with the artificial vagina. The sperm was frozen using two diluents (commercial AndroMed) and (commercial AndroMed, citric acid, gentamicin and glycerol). The semen had the following characteristics, volume 1.5±0.7 mL, viscosity 5.9±1.5, pH 7.1, with an alkalinity tendency and the color that predominated was the translucent white. The averages of kinetic parameters found in fresh semen were VCL (25.8±4.2 µm/s), VSL (10.3±2.5 µm/s) and VAP (18.9±3.2 µm/s). Kinetic parameters in cryopreserved semen showed that dilutor 2 VCL (8.4±1.4 µm/s), dilutor 1 (7.2±1.2 µm/s), VSL dilutor 2 (3.4±0.8 µm/s), dilutor 1 (2.9±0.7µm/s), VAP dilutor 2 (5.8±1.0 µm/s), dilutor 1 (4.8±0.8 µm/s). It is concluded that dilutor 2 is another alternative for cryopreservation of alpaca semen.
Description
Vol. 7, No. 1