Efectos de bencilaminopurina y tipo de brotes en la producción y calidad de plántulas de plátano vía macropropagación
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Rev. Inv. Cs. Agro. y Vet.
Abstract
La producción de plántulas de plátano de calidad vía macropropagación en cámara térmica es una metodología económica y eficiente para pequeños productores. El objetivo de la investigación fue evaluar el efecto de bencilaminopurina y tipo de brotes sobre la tasa de multiplicación de cormos y la calidad de plántulas de plátano propagadas en cámara térmica. Se desarrollaron dos experimentos separados. En el primero se probó el efecto de bencilaminopurina (BAP) sobre la tasa de multiplicación de cormos de plátano en cámara térmica, donde se establecieron dos tratamientos que fueron: Con BAP (40 mg L-1) y sin BAP. En el segundo se evaluó el potencial de enraizamiento, crecimiento y calidad de plántulas de plátano en vivero, generadas a partir de dos tipos de brotes en cámara térmica, que fueron: Brotes adventicios de segunda generación (BAR2) y brotes procedentes de tejido calloso (BCR2). En ambos experimentos los datos fueron analizados con prueba estadística de t de Student para observaciones pareadas. Se registró la tasa de multiplicación, variables de crecimiento y calidad de plántulas en vivero. La mayor tasa de multiplicación de brotes en cámara térmica se produjo con BAP, que supero en 44 % al tratamiento sin BAP. El mayor crecimiento e índice de calidad de Dickson en plántulas durante la aclimatación fue logrado con brotes adventicios (BAR2). La calidad se correlacionó positivamente con variables morfométricas de las plántulas. El uso de BAP y brotes adventicios (BAR2) es efectivo para incrementar tasa de multiplicación y calidad de plántulas de plátano macropropagadas.
The production of quality plantain seedlings way macropropagation in a thermal chamber is an economical and efficient methodology for small producers. The aim of the research was to evaluate the effect of benzylaminopurine and type of sprouts on the multiplication rate of corms and the quality of plantain seedlings propagated in a thermal chamber. Two separate experiments were performed. 1) To test the effect of benzylaminopurine (BAP) on the multiplication rate of plantain corms in a thermal chamber, two treatments were established were: With BAP (40 mg L-1) and without BAP. 2) To test the rooting ability, growth and seedlings quality plantain in the nursery phase generated from two types of sprouts in a thermal chamber that were: Second-generation adventitious sprouts (ASR2) and sprouts from callus tissue (CSR2). In both experiments, the data were analyzed with the Student's t test for paired observations. The multiplication rate, growth variables and quality of seedlings in the nursery were recorded. The highest rate of multiplication of sprout in thermal chamber occurred with BAP, which was 44% higher than the treatment without BAP. The highest growth and quality index of Dickson in seedlings during acclimatization was achieved with adventitious sprout (ASR2). The quality was positively correlated with morphometric variables of the seedlings. The use of BAP and adventitious sprout (ASR2) is effective in increasing the multiplication rate and quality of macropropagated plantain seedlings.
The production of quality plantain seedlings way macropropagation in a thermal chamber is an economical and efficient methodology for small producers. The aim of the research was to evaluate the effect of benzylaminopurine and type of sprouts on the multiplication rate of corms and the quality of plantain seedlings propagated in a thermal chamber. Two separate experiments were performed. 1) To test the effect of benzylaminopurine (BAP) on the multiplication rate of plantain corms in a thermal chamber, two treatments were established were: With BAP (40 mg L-1) and without BAP. 2) To test the rooting ability, growth and seedlings quality plantain in the nursery phase generated from two types of sprouts in a thermal chamber that were: Second-generation adventitious sprouts (ASR2) and sprouts from callus tissue (CSR2). In both experiments, the data were analyzed with the Student's t test for paired observations. The multiplication rate, growth variables and quality of seedlings in the nursery were recorded. The highest rate of multiplication of sprout in thermal chamber occurred with BAP, which was 44% higher than the treatment without BAP. The highest growth and quality index of Dickson in seedlings during acclimatization was achieved with adventitious sprout (ASR2). The quality was positively correlated with morphometric variables of the seedlings. The use of BAP and adventitious sprout (ASR2) is effective in increasing the multiplication rate and quality of macropropagated plantain seedlings.
Description
Vol. 5, No. 15