Evaluación de la actividad antiparasitaria de los extractos de 4 especies vegetales "chagasín" sobre trypanosoma cruzi procedentes de (triatomas infestans)" y su acción fitoterápica en cobayos chagásicos
Abstract
El presente estudio en esta etapa, tuvo como objetivo: Evaluar la actividad antiparasitaria de 4 extractos vegetales sobre Tripanosoma cruzi, aislados de Triatoma infestans y su acción fitoterápica en cobayos chagásicos. En la primera fase fue desarrollado el estudio de acuerdo a los objetivos específicos trazados, la metodología empleada estuvo basada en: 1. Obtención de los extractos vegetales por percolación, previo tratamiento de selección y desinfección de las especies en estudio; Jamillo, Carqueja, Canchalagua, los cuales previas obtenciones del extracto a diferentes concentraciones fueron sometidas sobre tripanosomas conservados en solución fisiológica isotónica a 28 +- 4 °C. los resultados para Jamillo demostraron mayor efecto antiparasitario en relación a las otras especies vegetales los parásitos vistas al microscopio tuvieron una inmovilidad pos mortuoria del 50% a las horas 8 horas y del 90% a las 16, y 99% a las 12 horas; las variables en estudio fueron concentración de los extractos y Tiempo de exposición del tripomastigote en las diferentes diluciones preliminares de los extractos semisólidos preparados al 0,4, 0,8, y 1,6 %. Los datos obtenidos fueron expresados como media ± desviación standar y las comparaciones entre los grupos experimentales fueron realizadas por ANOVA de una vía seguido, por una prueba post-hoc de Scheffé. Las medias fueron consideradas significativamente diferentes cuando p < 0,05. Se utilizó el software SPSS 11,5. De acuerdo al análisis de datos se han obtenido los siguientes resultados, sometidos a ANOVA, para todos los casos, existe un efecto significativo como se puede ver: probabilidad inferior a 0.05, por lo que existen diferencias significativas entre los tratamientos aplicados. Concluyéndose que los extractos de las especies utilizadas Schkuhria pinnata (Canchalagua) y Baccharis articulata (Carqueja), Miroxylum periferium (Quina colorada) y Viscum álbum L. (Jamillo), demostraron actividad tripanocida en menor y mayor grado de acuerdo a las diferentes concentraciones utilizadas en el ensayo, asimismo en el ensayo biológico de cobayos chagásicos, desparasitados con CHAGASIN l(extracto de Jamillo estabilizado con extracto de carqueja) se demostró mayor actividad antiparasitaria que CHAGASIN 2 (extracto de Jamillo estabilizado con canchalagua)
The aim of the present study on its first stage was to assess the anti-parasitic activity of four plant extracts on Trypanosomacruziand isolated from Triatomainfestants and its phytotherapeutic action in chagasic guinea pigs. In the first phase the study was developed according to the specific objectives set. The methodology used was based on: 1 .Obtaining plant extracts by percolation after a treatment of selection and disinfection of the species under study: Jamillo, Carqueja, Canchagula, which previous obtained of the extract at different concentration were put on preserved trypanosomes in a physiological isotonic solution at 28 +- 4°C. The results for Jamillo showed greater antiparasitic effects in relation to the other plant species. The parasites seen under microscope had a post-mortuary immobility of 50 % at 8 hours, 90 % at 16 and 99 % at 12. The variables under study were concentration of the extracts and time of exposure of the trypomastigote in the different preliminary dilutions of the semisolid extracts prepared at 0.4, 0.8 and 1.6 %. The data obtained was expressed as a mean +-standard deviation and the comparisons between the experimental groups were performed by one-way ANOVA followed by a post hoc Scheffé test using the SPSS 11.5 software. The means were considered significantly different when p<0.05. The following results were obtained according to the data analysis and treatment submitted to ANOVA. For all cases, there is a significant effect: probability less than 0.05, so there are significant differences among the treatments applied. Therefore, it can be concluded that the extracts of the species used: Schkuhria pinnata (Canchalagua), Baccharis articulata (Carqueja), Miroxylum periferium (Quina Colorada) and Viscum álbum L. (Jamillo), showed trypanocidal activity in minor and greater degree according to the different concentrations used in the lab experiments. The same was observed in the biological test of chagasic guinea pigs, wormed whit CHAGASIN 1 (extract of Jamillo stabilized with extract of carqueja). It showed greater antiparasitic activity than CHAGASIN 2 (extract of Jamillo stabilized with canchagula).
The aim of the present study on its first stage was to assess the anti-parasitic activity of four plant extracts on Trypanosomacruziand isolated from Triatomainfestants and its phytotherapeutic action in chagasic guinea pigs. In the first phase the study was developed according to the specific objectives set. The methodology used was based on: 1 .Obtaining plant extracts by percolation after a treatment of selection and disinfection of the species under study: Jamillo, Carqueja, Canchagula, which previous obtained of the extract at different concentration were put on preserved trypanosomes in a physiological isotonic solution at 28 +- 4°C. The results for Jamillo showed greater antiparasitic effects in relation to the other plant species. The parasites seen under microscope had a post-mortuary immobility of 50 % at 8 hours, 90 % at 16 and 99 % at 12. The variables under study were concentration of the extracts and time of exposure of the trypomastigote in the different preliminary dilutions of the semisolid extracts prepared at 0.4, 0.8 and 1.6 %. The data obtained was expressed as a mean +-standard deviation and the comparisons between the experimental groups were performed by one-way ANOVA followed by a post hoc Scheffé test using the SPSS 11.5 software. The means were considered significantly different when p<0.05. The following results were obtained according to the data analysis and treatment submitted to ANOVA. For all cases, there is a significant effect: probability less than 0.05, so there are significant differences among the treatments applied. Therefore, it can be concluded that the extracts of the species used: Schkuhria pinnata (Canchalagua), Baccharis articulata (Carqueja), Miroxylum periferium (Quina Colorada) and Viscum álbum L. (Jamillo), showed trypanocidal activity in minor and greater degree according to the different concentrations used in the lab experiments. The same was observed in the biological test of chagasic guinea pigs, wormed whit CHAGASIN 1 (extract of Jamillo stabilized with extract of carqueja). It showed greater antiparasitic activity than CHAGASIN 2 (extract of Jamillo stabilized with canchagula).
Description
Vol. 15, No. 16