Efecto de la exposición prenatal al paraquat (gramoxone® n.f.) en ratas biou: wistar. Estudio preliminar Effect of prenatal exposure to paraquat (gramoxone® n.f.) in biou rats: wistar. Preliminary study

Abstract

The aim of the research was to determine the effect of prenatal exposure to the paraquat (PQ) on craniofacial and body conformation in BIOU rats: Wistar. Methodology: fifty pregnant rats were distributed in five groups: PQ1 and PQ2, respectively received 2 and 3 mg / kg of PQ via subcutaneous (s.c); vitamin A (positive control): treated with 100,000 IU of vitamin A intragastric route (i.g) and control groups (distilled water, s.c route and i.g route). The treatments were administered from 6th to the 15th day of gestation. On days 6, 14 and 21 the maternal weight was compared. On the last day, a laparotomy was performed and the number of implantations, reabsorptions, live and dead fetuses, fetal weight and height, and the presence of craniofacial and body alterations were determined. Results: there was no significant difference in maternal weight gain between the groups. In the PQ 2mg / Kg and vitamin A groups, the highest number of dead fetuses and reabsorptions were respectively registered. The fetuses weight and height in the vitamin A group was lower (p <0.05) than the rest. The PQ 2 mg / kg group presented a greater number of body malformations. In vitamin A group macroglossia, cleft lip and generalized edema were observed. Conclusion: The presence of body malformations was demonstrated in fetuses exposed during the prenatal period to PQ, showing a teratogenic effect, not dependent on the dose, in the BIOU: Wistar line of rats. It is recommended to increase the number of pregnant rats, to evaluate these results in future research.