Aislamiento y caracterización de bacterias PGPR asociadas con ají morrón (Capsicum annum Group) en invernadero
Date
Journal Title
Journal ISSN
Volume Title
Publisher
RIIARn
Abstract
El uso de bacterias promotoras del crecimiento vegetal (PGPR) representa una alternativa sostenible en la producción agrícola, en especial rubros con exigencias internacionales sobre prácticas sostenibles. El pimiento producido en invernaderos de República Dominicana, representa uno de los cultivos de mayor crecimiento y rentabilidad en las últimas décadas. La investigación tuvo el objetivo de aislar, caracterizar bacterias PGPR y evaluar el potencial efecto bioestimulante en el cultivo de pimiento. Se colectaron muestras de raíces de plantas sanas de pimiento producidos en invernaderos de las provincias Azua, San José de Ocoa y La Vega, República Dominicana. Las bacterias endofíticas fueron aisladas, purificadas e identificadas mediante extracción de ADN, secuenciación del gen 16S rRNA, comparadas en el banco Gene Bank y se realizaron pruebas bioquímicas. Se aislaron 117 bacterias mediante secuenciación molecular de las cuales ocho resultaron ser PGPR. Entre estas se encuentran: Bacillus siamensis, Pseudomonas atacamensis, Rhizobium oryzihabitans, Bacillus haikouensis, Antribacter gilvus, Enterobacter ludwigii, Priestia aryabhattai y Kosakonia pseudosacchari. Estas bacterias crecieron a diferentes rangos de temperaturas, pH y salinidad. Seis fueron positivas a catalasa y una produjo sideróforos. En pruebas en invernadero, con un diseño en bloques al azar, se inocularon plantas de pimiento para evaluar altura, grosor del tallo y biomasa. Las bacterias mostraron diferencias significativas en relación al testigo sin fertilizar ni inocular. Aunque no hubo diferencias significativas con el testigo fertilizado, se observó un efecto positivo, incrementando el desarrollo de las plantas. Los resultados sugieren que especies como Bacillus siamensis tienen potencial como biofertilizantes por su adaptación a las condiciones del medio y por su efecto bioestimulante en las plantas.
The use of plant growth promoting rhyzobacteria (PGPR) represents a sustainable alternative in agricultural production, especially in areas with international requirements for sustainable practices. Peppers produced in greenhouses in the Dominican Republic are one of the fastest-growing and most profitable crops in recent decades. The research aimed to isolate and characterize PGPR bacteria and evaluate their potential biostimulant effect on pepper crops. Root samples were collected from healthy pepper plants produced in greenhouses in the provinces of Azua, San José de Ocoa, and La Vega, Dominican Republic. Endophytic bacteria were isolated, purified, and identified by DNA extraction, 16S rRNA gene sequencing, and compared with Gene Bank data. Biochemical tests were performed. One hundred and seventy-seven bacteria were isolated by molecular sequencing, eight of which were found to be PGPR. These included Bacillus siamensis, Pseudomonas atacamensis, Rhizobium oryzihabitans, Bacillus haikouensis, Antribacter gilvus, Enterobacter ludwigii, Priestia aryabhattai, and Kosakonia pseudosacchari. These bacteria grew at different temperatures, pH, and salinity levels. Six were catalase-positive, and one produced siderophores. In greenhouse tests using a randomized block design, pepper plants were inoculated to evaluate height, stem thickness, and biomass. The bacteria showed significant differences compared to the unfertilized and uninoculated control. Although there were no significant differences compared to the fertilized control, a positive effect was observed, increasing plant development. The results suggest that species such as Bacillus siamensis have potential as biofertilizers due to their adaptation to environmental conditions and their biostimulating effect on plants.
The use of plant growth promoting rhyzobacteria (PGPR) represents a sustainable alternative in agricultural production, especially in areas with international requirements for sustainable practices. Peppers produced in greenhouses in the Dominican Republic are one of the fastest-growing and most profitable crops in recent decades. The research aimed to isolate and characterize PGPR bacteria and evaluate their potential biostimulant effect on pepper crops. Root samples were collected from healthy pepper plants produced in greenhouses in the provinces of Azua, San José de Ocoa, and La Vega, Dominican Republic. Endophytic bacteria were isolated, purified, and identified by DNA extraction, 16S rRNA gene sequencing, and compared with Gene Bank data. Biochemical tests were performed. One hundred and seventy-seven bacteria were isolated by molecular sequencing, eight of which were found to be PGPR. These included Bacillus siamensis, Pseudomonas atacamensis, Rhizobium oryzihabitans, Bacillus haikouensis, Antribacter gilvus, Enterobacter ludwigii, Priestia aryabhattai, and Kosakonia pseudosacchari. These bacteria grew at different temperatures, pH, and salinity levels. Six were catalase-positive, and one produced siderophores. In greenhouse tests using a randomized block design, pepper plants were inoculated to evaluate height, stem thickness, and biomass. The bacteria showed significant differences compared to the unfertilized and uninoculated control. Although there were no significant differences compared to the fertilized control, a positive effect was observed, increasing plant development. The results suggest that species such as Bacillus siamensis have potential as biofertilizers due to their adaptation to environmental conditions and their biostimulating effect on plants.
Description
Vol. 12, No. 2