<b> <i>Leishmania</i> Antigen Nonspecific Binding to Avidin in Biotin-Avidin Immunoassays </b>

Abstract

One of the most prevalent uses of avidin-biotin technology in recent years has been their use in immunoassays. In many cases, the signal is enhanced 10-fold or more, owing to the four biotin-binding sites of avidin and the multiple biotinyl groups on the derivatized antibody ([4][1], [5][2]). This