Especies de lactobacillus potencialmente probióticos aisladas de cavidad bucal de niños nacidos por parto natural.

Abstract

Recently, the vertical transmission of the usual microbiota from mother to child during pregnancy has been stablished. However, since ancient times it has been accepted that during the passage through the birth canal the fetus had its first microbiological contact and was inoculated with the vaginal microbiota of the mother. Within the vaginal microbial universe coexist about 300 species of bacteria, Lactobacillus being the predominant genus, which performs protective probiotic functions. The objective of the present research consisted in verifying the presence of Lactobacillus spp. in oral samples of 17 neonates born by natural birth in the Gynecology-Obstetrics Unit of Hospital Sor Juana Ines de la Cruz, Merida-Venezuela during the third quarter of 2015. Prior consent of the parents and endorsement of the Hospital Directive, the sample was collected by swab of the cheeks, gums and floor of the mouth, inoculated in MRS broth and incubated at 37 °C for 48 hours in microaerobiosis. Subsequently, isolation was carried out on MRS agar under the same incubation conditions. Typical colonies were selected macroscopically, microscopic characteristics were evaluated, as well as preliminary tests for the genus Lactobacillus, obtaining 49 isolates. Six isolates were selected by biochemical identification resulting in 2 Lactobacillus acidophilus, 1 Lactobacillus plantarum, 2 Lactobacillus paracaseissp. paracasei and 1 Lactobacillus fermentum species widely recognized as probiotics. The findings obtained demonstrate the presence of Lactobacilli in the neonatal oral cavity and contribute to studies related to vertical transmission mother-child.