Capacidad biocontroladora de Beauveria brongniartii (Sacc.) y Metarhizium anisopliae (Metsch.,) en el control de pulgones Macrosiphum euphorbiae (Hemiptera: Aphididae)
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J. Selva Andina Res. Soc.
Abstract
Con el interés de desarrollar tecnologías alternativas en el control de pulgones (Macrosiphum euporviae), se evaluó la capacidad biocontroladora de los hongos entomopatógenos Beauveria brongniartii y Metarhizium anisopliae. Par el estudio se colectó hongos nativos de 3 partes del altiplano central (Achacachi, Viacha y Patacamaya), se aislaron de insectos momificados y purificaron en laboratorio, los aislados dieron como resultado hongos pertenecientes al género Beauveria sp., y Metarhizium sp. De estos aislados se sacó su concentración inicial y todos fueron ajustados a la mínima concentración presentada en uno de los aislados que fue de 9.5 x 10⁴ conidios/mL con estas concentraciones se realizó la prueba de virulencia durante 144 h, los aislados 2BbVch, 1BbAch, 1MtAch y 3BbPtm produjeron más del 50% de mortalidad, indicando que los aislados del genero Beauveria resultaron ser mejores. Con los aislados que demostraron mejor mortalidad se los sometió a una segunda prueba de la determinación del tiempo letal 50 y 80 (Tl50), (Tl80) en donde el aislado 2BbVch demostró un menor tiempo en controlar el 50 y 80% de afidos con 78.1 y 123.8 h respectivamente. Se determinó la concentración letal 50 y 80 (Cl50), (Cl80), en donde se pudo verificar que el aislado 2BbVch y 1BbAch requieren concentraciones mínimas de 2.5x10⁴ y 5.1x10⁴ conidias/mL, para controlar el 50% de pulgones y a una concentración de 1.3x10(5) y 1.8x10(5) conidias/mL para controlar 80% de pulgones.
Aiming to develop alternative technologies in controlling aphids (Macrosiphum euporviae), the biocontrol ability of entomopathogenic fungi Beauveria brongniartii and Metarhizium anisopliae evaluated. Par study native fungi of 3 parts of the central highlands (Achacachi , Viacha and Patacamaya) , which is isolated from the mummified insects and purified in the laboratory, the isolates were as fungi of the genus Beauveria result and Metarhizium were collected. Of these isolates its initial concentration was removed and all were adjusted to the minimum concentration lodged in one of the isolates was 9.5 x 10⁴conidia/mL at these concentrations the virulence test was performed for 144 hours , where isolated 2BbVch , 1BbAch , 3BbPtm 1MtAch and produced more than 50 % mortality , indicating that the genus Beauveria isolates proved to be better . With isolates that demonstrated improved mortality were subjected to a second test determining the lethal time 50 and 80 ( LT50 ) ( TL80 ) where 2BbVch isolated showed a lower time control 50 and 80% with 78 aphids 1 and 123.8 hours respectively . 50 and the lethal concentration 80 ( IC50 ) was determined, ( IC80 ), where it was observed that the isolated 1BbAch 2BbVch and require minimal concentrations of 2.5 × 10⁴ and 5.1 x 10⁴ conidia/mL, to control 50% of aphids as a concentration of 1.3 x 10(5) to 1.8 x10(5) conidia/mL for 80 % control of aphids.
Aiming to develop alternative technologies in controlling aphids (Macrosiphum euporviae), the biocontrol ability of entomopathogenic fungi Beauveria brongniartii and Metarhizium anisopliae evaluated. Par study native fungi of 3 parts of the central highlands (Achacachi , Viacha and Patacamaya) , which is isolated from the mummified insects and purified in the laboratory, the isolates were as fungi of the genus Beauveria result and Metarhizium were collected. Of these isolates its initial concentration was removed and all were adjusted to the minimum concentration lodged in one of the isolates was 9.5 x 10⁴conidia/mL at these concentrations the virulence test was performed for 144 hours , where isolated 2BbVch , 1BbAch , 3BbPtm 1MtAch and produced more than 50 % mortality , indicating that the genus Beauveria isolates proved to be better . With isolates that demonstrated improved mortality were subjected to a second test determining the lethal time 50 and 80 ( LT50 ) ( TL80 ) where 2BbVch isolated showed a lower time control 50 and 80% with 78 aphids 1 and 123.8 hours respectively . 50 and the lethal concentration 80 ( IC50 ) was determined, ( IC80 ), where it was observed that the isolated 1BbAch 2BbVch and require minimal concentrations of 2.5 × 10⁴ and 5.1 x 10⁴ conidia/mL, to control 50% of aphids as a concentration of 1.3 x 10(5) to 1.8 x10(5) conidia/mL for 80 % control of aphids.
Description
Vol. 8, No. 1